AJP - Regulatory, Integrative and Comparative Physiology, Vol 232, Issue 1 54-R59, Copyright © 1977 by American Physiological Society

ARTICLES

Total body water and the exchangeable hydrogen. I. Theoretical calculation of nonaqueous exchangeable hydrogen in man

J. M. Culebras and F. D. Moore

A theoretical calculation of the total nonaqueous exchangeable hydrogen in protein, carbohydrates, and fat in man has been made. It shows that of the total exchangeable hydrogen in the body 5.22% is located in biochemical components, soluble in body water, containing hydrogen that is exchangeable with the isotope. This value represents a maximum upward distortion of total body water measurements by isotope dilution, due to the maximum possible exchangeability in these molecular conformations. From comparative measurements reported in the literature it is clear that this maximum is not achieved during the short period of time during which tritium-dilution studies are performed. It is the authors' belief that the hard-to-exchange amide hydrogens described by Blout in the protein conformations account for this failure of the isotope to achieve complete exchange in the short time allowed.

This article has been cited by other articles:

				S. Salinari, A. Bertuzzi, M. Manco, and G. Mingrone NEFA-glucose comodulation model of beta-cell insulin secretion in 24-h multiple-meal test Am J Physiol Endocrinol Metab, June 1, 2007; 292(6): E1890 - E1898. [Abstract] [Full Text] [PDF]

				A. V. Greco, G. Mingrone, A. Giancaterini, M. Manco, M. Morroni, S. Cinti, M. Granzotto, R. Vettor, S. Camastra, and E. Ferrannini Insulin Resistance in Morbid Obesity: Reversal With Intramyocellular Fat Depletion Diabetes, January 1, 2002; 51(1): 144 - 151. [Abstract] [Full Text] [PDF]

				T. Hedestig, A. Ebberyd, and S. G. E. Lindahl Validation of equilibration and chromium reduction methods for deuterium measurements of fluid volumes J Appl Physiol, August 1, 2001; 91(2): 733 - 736. [Abstract] [Full Text] [PDF]

				C. J. de Souza, M. Eckhardt, K. Gagen, M. Dong, W. Chen, D. Laurent, and B. F. Burkey Effects of Pioglitazone on Adipose Tissue Remodeling Within the Setting of Obesity and Insulin Resistance Diabetes, August 1, 2001; 50(8): 1863 - 1871. [Abstract] [Full Text] [PDF]

				A. C. JOHANSSON, O. SAMUELSSON, P. O. ATTMAN, I. BOSAEUS, and B. HARALDSSON Limitations in Anthropometric Calculations of Total Body Water in Patients on Peritoneal Dialysis J. Am. Soc. Nephrol., March 1, 2001; 12(3): 568 - 573. [Abstract] [Full Text]

				G. Hartnoll, P. Bétrémieux, and N. Modi Body water content of extremely preterm infants at birth Arch. Dis. Child. Fetal Neonatal Ed., July 1, 2000; 83(1): 56F - 59. [Abstract] [Full Text]

				Z. Wang, P. Deurenberg, W. Wang, A. Pietrobelli, R. N Baumgartner, and S. B Heymsfield Hydration of fat-free body mass: review and critique of a classic body-composition constant Am. J. Clinical Nutrition, May 1, 1999; 69(5): 833 - 841. [Abstract] [Full Text] [PDF]

				J. W Mott, J. Wang, J. C Thornton, D. B Allison, S. B Heymsfield, and R. N Pierson Jr Relation between body fat and age in 4 ethnic groups Am. J. Clinical Nutrition, May 1, 1999; 69(5): 1007 - 1013. [Abstract] [Full Text] [PDF]

				G. Gotaas, E. Milne, P. Haggarty, and N. J. C. Tyler Use of feces to estimate isotopic abundance in doubly labeled water studies in reindeer in summer and winter Am J Physiol Regulatory Integrative Comp Physiol, October 1, 1997; 273(4): R1451 - R1456. [Abstract] [Full Text] [PDF]