AJP - Regulatory, Integrative and Comparative Physiology, Vol 242, Issue 1 77-R84, Copyright © 1982 by American Physiological Society

ARTICLES

Thermosensitive single-unit activity of in vitro hypothalamic slices

S. R. Kelso, M. N. Perlmutter and J. A. Boulant

Single-unit activity was recorded in vitro from tissue slices of rat preoptic area-anterior hypothalamus. The thermosensitivity of 139 units was determined by their changes in firing rate in response to changes in slice temperature. Of these neurons, 30% were warm sensitive, 10% were cold sensitive, and 60% were temperature insensitive. These proportions are similar to results obtained in whole-animal studies, indicating that this is a viable preparation. It also suggests that hypothalamic neuronal thermosensitivity is not dependent on peripheral afferent input. All units had low firing rates (less than 10 imp/s) at 37 degrees C, and 83% of the warm-sensitive units were most thermosensitive above 37 degrees C. This supports the concept that afferent input determines the level of firing rate and range of thermosensitivity of warm-sensitive neurons. The cold-sensitive units also displayed maximal thermosensitivity above 37 degrees C, which would be expected if cold-sensitive neurons received inhibitory synaptic input from nearby warm-sensitive neurons.

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