AJP - Regulatory, Integrative and Comparative Physiology, Vol 242, Issue 5 570-R576, Copyright © 1982 by American Physiological Society
Perfused rat hindlimb wound model: lambda-carrageenan induced
J. A. O'Connor, R. L. Scott, P. W. Mellick and M. D. Caldwell
We established an isolated perfused rat hindlimb wound model using
intramuscular injections of lambda-carrageenan. Wound and control hindlimbs
of 24-h fasting anesthetized (phenobarbital sodium, 5 mg/100 g body wt)
rats were perfused for 60 min (10 ml/min) with media containing 10 mM
glucose and physiological levels of amino acids. Analysis of perfusate
showed a significant increase in glucose clearance and lactate production
from the wounded hindlimb but no significant difference in tissue adenine
nucleotide levels. Alterations in net amino acid release were measured over
a 60-min period; all amino acids were released in greater concentration
from wounded hindlimbs. Comparison of amino acid release, normalized for
phenylalanine concentration, suggested the following alterations in amino
acid metabolism by the wounded tissue: depressed threonine, glycine serine
and alanine release, and increased release of the branched chain amino
acids (BCAA), leucine, isoleucine, and valine. The increased release and
intracellular concentration of BCAA was supported by decreased BCAA
transaminase activity in the wounded muscle tissue. This result may explain
the decreased alanine release from the wounded hindlimbs.
