AJP - Regulatory, Integrative and Comparative Physiology, Vol 246, Issue 3 325-R330, Copyright © 1984 by American Physiological Society
Changes in glycerol kinetics after E. coli endotoxin administration in dogs
O. P. McGuinness and J. J. Spitzer
The metabolic clearance rate (MCR) and maximal rate of glycerol removal (Rd
max) were determined in anesthetized dogs at two time periods after the
intravenous administration of either Escherichia coli endotoxin or saline.
The nonisotopic method employed in these studies to determine the MCR of
glycerol consisted of a constant infusion of glycerol at three different
infusion rates. At each infusion rate a steady-state glycerol concentration
was obtained. The reciprocal of the slope of the linear relationship
between the glycerol infusion rate and the change in the steady-state
arterial glycerol concentration was equal to the MCR of glycerol.
Administration of endotoxin significantly decreased the MCR of glycerol,
whereas the volume of distribution of glycerol was not altered
significantly. The arterial glycerol turnover remained unaltered, whereas
arterial glycerol concentration increased after endotoxin administration.
The studies demonstrate that the elevated arterial glycerol concentration
maintained the rate of glycerol turnover in the face of decreased
efficiency of glycerol removal after endotoxin administration.
