AJP - Regulatory, Integrative and Comparative Physiology, Vol 246, Issue 4 471-R478, Copyright © 1984 by American Physiological Society
Influence of thermal acclimation on glucose production and ketogenesis in isolated eel hepatocytes
D. Jankowsky, W. Hotopp and H. Seibert
Hepatocytes were isolated by collagenase perfusion of the liver from adult
eels (Anguilla anguilla L.) acclimated to different temperatures. Whereas
the relative weight of the liver increased in cold-acclimated fish,
hepatocytes from 10- and 20 degrees C-acclimated animals did not differ in
cellular weight, dry weight, or protein content. Endogenous rates of oxygen
consumption and respiratory control ratios were independent of acclimation
temperature. There was no effect of temperature on triacylglycerol content,
but glycogen concentration was significantly higher in hepatocytes of
cold-acclimated fish. Liver cells from cold-acclimated eels exhibited
higher rates of glucose release and ketogenesis than those from
warm-acclimated animals. It is concluded that the increase in acetoacetate
production induced by cold acclimation results primarily from a higher rate
of lipolysis. Cellular interactions between ketogenesis and gluconeogenesis
are demonstrated and discussed.
