AJP - Regulatory, Integrative and Comparative Physiology, Vol 252, Issue 2 276-R283, Copyright © 1987 by American Physiological Society
Time course of radiolabeled 2-deoxy-D-glucose 6-phosphate turnover in cerebral cortex of goats
D. A. Pelligrino, D. J. Miletich and R. F. Albrecht
The in vivo dephosphorylation rate of 2-deoxy-D-glucose 6-phosphate (DGP)
in the cerebral cortex of goats injected intravenously with radiolabeled
2-deoxy-D-glucose (DG) was investigated. Serial rapidly frozen samples of
parietal cortical gray tissue were obtained at regular intervals over time
periods from 45 min to 3 h in awake goats or in paralyzed and artificially
ventilated goats maintained under 70% N2O or pentobarbital sodium
anesthesia. The samples were analyzed for glucose content and separate DG
and DGP activities. The rate parameters for phosphorylation (k3*) and
dephosphorylation (k4*) were estimated in each animal. The glucose
phosphorylation rate (PR) was calculated over the intervals 3-5 (or 6),
3-10, 3-20, 3-30, and 3-45 min, assuming k4* = 0. As the evaluation period
was extended beyond 10 min, the calculated PR became increasingly less when
compared with that calculated over the 3- to 5- (or 6) min interval (PRi).
Furthermore, as metabolic activity decreased, the magnitude of the error
increased such that at 45 min pentobarbital-anesthetized goats
underestimated the PRi by 46.5% compared with only 23.1% in
N2O-anesthetized goats. This was also reflected in the greater than twofold
higher k4*/k3* ratio in the pentobarbital vs. N2O-anesthetized group. It is
concluded that when using the DG method in the goat, DGP dephosphorylation
cannot be ignored when employing greater than 10-min evaluation periods.
