AJP - Regu AJP: Advances in Physiology Education
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Regul Integr Comp Physiol 254: R491-R498, 1988;
0363-6119/88 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Perry, S. F.
Right arrow Articles by Flik, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Perry, S. F.
Right arrow Articles by Flik, G.

AJP - Regulatory, Integrative and Comparative Physiology, Vol 254, Issue 3 491-R498, Copyright © 1988 by American Physiological Society

ARTICLES

Characterization of branchial transepithelial calcium fluxes in freshwater trout, Salmo gairdneri

S. F. Perry and G. Flik
Department of Biology, University of Ottawa, Ontario, Canada.

Experiments were performed to determine whether gill transepithelial calcium fluxes in the freshwater trout (Salmo gairdneri) are passive or require active transport and to characterize the mechanisms involved. A comparison of the in vivo unidirectional flux ratios with the flux ratios calculated according to the transepithelial electrochemical gradients revealed that calcium uptake from the water requires active transport of Ca2+. The inhibition of calcium uptake by external lanthanum, the specific deposition of lanthanum on the apical surface of chloride cells, and the favorable electrochemical gradient for calcium across the apical membrane suggest that the initial step in branchial calcium uptake is the passive entry of calcium into the cytosol of chloride cells through apical channels that are permeable to calcium. The study of gill basolateral plasma membrane vesicles demonstrated the existence of a high-affinity calmodulin-dependent calcium-transporting system [half-maximal Ca2+ concentration (K0.5) = 160 nM, Vmax = 1.86 nmol.min-1.mg protein-1]. This system actively transports calcium from the cytosol of chloride cells into the plasma against a sizeable electrochemical gradient, thereby completing the transepithelial uptake of calcium. Calcium efflux occurs passively through paracellular pathways between chloride cells and adjacent pavement cells or between neighboring pavement cells.


This article has been cited by other articles:


Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
T. M. Rodela, J. S. Ballantyne, and P. A. Wright
Carrier-mediated urea transport across the mitochondrial membrane of an elasmobranch (Raja erinacea) and a teleost (Oncorhynchus mykiss) fish
Am J Physiol Regulatory Integrative Comp Physiol, June 1, 2008; 294(6): R1947 - R1957.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
P. M. Guerreiro, J. L. Renfro, D. M. Power, and A. V. M. Canario
The parathyroid hormone family of peptides: structure, tissue distribution, regulation, and potential functional roles in calcium and phosphate balance in fish
Am J Physiol Regulatory Integrative Comp Physiol, February 1, 2007; 292(2): R679 - R696.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
J. S. Bystriansky and J. S. Ballantyne
Gill Na+-K+-ATPase activity correlates with basolateral membrane lipid composition in seawater- but not freshwater-acclimated Arctic char (Salvelinus alpinus)
Am J Physiol Regulatory Integrative Comp Physiol, February 1, 2007; 292(2): R1043 - R1051.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
A. Shahsavarani and S. F. Perry
Hormonal and environmental regulation of epithelial calcium channel in gill of rainbow trout (Oncorhynchus mykiss)
Am J Physiol Regulatory Integrative Comp Physiol, November 1, 2006; 291(5): R1490 - R1498.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
T.-C. Pan, B.-K. Liao, C.-J. Huang, L.-Y. Lin, and P.-P. Hwang
Epithelial Ca2+ channel expression and Ca2+ uptake in developing zebrafish
Am J Physiol Regulatory Integrative Comp Physiol, October 1, 2005; 289(4): R1202 - R1211.
[Abstract] [Full Text] [PDF]

Home page
 J. Exp. Biol.Home page
N. R. Bury, P. A. Walker, and C. N. Glover
Nutritive metal uptake in teleost fish
J. Exp. Biol., January 1, 2003; 206(1): 11 - 23.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
G. A. Fines, J. S. Ballantyne, and P. A. Wright
Active urea transport and an unusual basolateral membrane composition in the gills of a marine elasmobranch
Am J Physiol Regulatory Integrative Comp Physiol, January 1, 2001; 280(1): R16 - R24.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
M. G. Wheatly, R. C. Pence, and J. R. Weil
ATP-dependent calcium uptake into basolateral vesicles from transporting epithelia of intermolt crayfish
Am J Physiol Regulatory Integrative Comp Physiol, February 1, 1999; 276(2): R566 - R574.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
M.-H. Chang, H.-C. Lin, and P.-P. Hwang
Ca2+ uptake and Cd2+ accumulation in larval tilapia (Oreochromis mossambicus) acclimated to waterborne Cd2+
Am J Physiol Regulatory Integrative Comp Physiol, June 1, 1998; 274(6): R1570 - R1577.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online