AJP - Regulatory, Integrative and Comparative Physiology, Vol 254, Issue 6 885-R890, Copyright © 1988 by American Physiological Society
Comparison of fibrinolytic activities of human and bovine endothelial cells
K. Bartha, J. Wojta, O. F. Wagner and B. R. Binder
Department of Medical Physiology, University of Vienna, Austria.
Human aortic (HAE), human umbilical vein (HUVE), and bovine aortic (BAE)
endothelial cells were compared in their synthesis and release of
fibrinolytic components during culturing. After isolation, the cultures
were grown to confluency and then studied under identical conditions for
release of tissue plasminogen activator (t-PA) antigen and plasminogen
activator inhibitor (PAI) into serum-free medium. HAE cells released 10
times more t-PA antigen than HUVE cells, and the respective cell lysates
also contained comparably higher values. Free PAI capacity was found in the
conditioned media of both HAE and HUVE cells. BAE cell t-PA release was
much lower than that of the HAE cells, and free inhibitor capacity was not
found in the conditioned medium. BAE cells contained significant amounts of
PA activity in cell membrane-bound form. This PA activity on the cell
surface was not stimulated by addition of CNBr fibrinogen fragments but
could be partially inhibited by activated bovine PAI and antibodies against
human t-PA and urokinase PA, respectively.
