AJP - Regulatory, Integrative and Comparative Physiology, Vol 255, Issue 2 289-R294, Copyright © 1988 by American Physiological Society

ARTICLES

Subcellular enzyme binding in glycolytic control: in vivo studies with fish muscle

S. P. Brooks and K. B. Storey
Institute of Biochemistry, Carleton University, Ottawa, Ontario, Canada.

The effect of exercise on the binding of glycolytic enzymes to subcellular structures was examined in rainbow trout (Salmo gardneri). Both "burst" and "endurance" type exercise produced an increase of approximately 50% in the percentage of phosphofructokinase (PFK), glyceraldehyde 3-phosphate dehydrogenase (GAPDH), aldolase, and 3-phosphoglycerate kinase associated with particulate matter in white skeletal muscle. In contrast, cardiac muscle showed no change in enzyme binding when trout were exercised, suggesting that the effects seen in white muscle are related to an increased anaerobic glycolytic flux in this tissue. Modulation of binding by altered pH or calcium ion concentration was tested: a decrease in pH increased PFK binding to subcellular particles, whereas 2 mM CaCl2 decreased GAPDH binding. These results are discussed with respect to the formation of a glycolytic complex during exercise in trout white muscle.

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