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Am J Physiol Regul Integr Comp Physiol 256: R572-R576, 1989;
0363-6119/89 $5.00
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AJP - Regulatory, Integrative and Comparative Physiology, Vol 256, Issue 2 572-R576, Copyright © 1989 by American Physiological Society


ARTICLES

Validation of single-sample doubly labeled water method

M. D. Webster and W. W. Weathers
Department of Avian Sciences, University of California, Davis 95616.

We have experimentally validated a single-sample variant of the doubly labeled water method for measuring metabolic rate and water turnover in a very small passerine bird, the verdin (Auriparus flaviceps). We measured CO2 production using the Haldane gravimetric technique and compared these values with estimates derived from isotopic data. Doubly labeled water results based on the one-sample calculations differed from Haldane values by less than 0.5% on average (range -8.3 to 11.2%, n = 9). Water flux computed by the single-sample method differed by -1.5% on average from results for the same birds based on the standard, two-sample technique (range -13.7 to 2.0%, n = 9).


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