AJP - Regulatory, Integrative and Comparative Physiology, Vol 258, Issue 2 332-R337, Copyright © 1990 by American Physiological Society

ARTICLES

Antiserum against tumor necrosis factor enhances lipopolysaccharide fever in rats

N. C. Long, S. L. Kunkel, A. J. Vander and M. J. Kluger
Department of Physiology, University of Michigan Medical School, Ann Arbor 48109.

The role of tumor necrosis factor (TNF, cachectin), a putative endogenous pyrogen, was investigated by comparing fever and plasma TNF levels after the intraperitoneal and intramuscular injection of 10 micrograms/kg lipopolysaccharide (LPS) into male Sprague-Dawley rats and by neutralization of endogenous TNF using TNF antiserum. An intraperitoneal injection of LPS caused a biphasic fever that lasted approximately 6.5 h. TNF levels in these rats peaked at 657 +/- 222 U/ml at 1 h then declined to virtually undetectable levels by the fourth hour. The intramuscularly injected animals showed a lower monophasic fever and low sustained TNF levels (40 +/- 10 U/ml at 1 h, 18 +/- 11 U/ml at 4 h). In a second study, an antiserum that had been shown to neutralize rat TNF was injected intraperitoneally 2 h before the intramuscular injection of 10 micrograms/kg LPS. Control rats were injected with normal rabbit serum before LPS. During the second hour after the injection of LPS, the animals that received the antiserum developed fevers that tended to be lower than those seen in the rats that were injected with control serum (0.33 +/- 0.06 vs. 0.58 +/- 0.1), although this difference was not significant. However, during the third through eighth hours after LPS, the antiserum-injected rats had mean body temperatures that were significantly higher than those of the control rats (1.62 +/- 0.11 vs. 1.07 +/- 0.09; P = 0.0005).(ABSTRACT TRUNCATED AT 250 WORDS)

This article has been cited by other articles:

				S. J. Spencer, A. Mouihate, M. A. Galic, and Q. J. Pittman Central and peripheral neuroimmune responses: hyporesponsiveness during pregnancy J. Physiol., January 15, 2008; 586(2): 399 - 406. [Abstract] [Full Text] [PDF]

				M.-E. Fortier, S. Kent, H. Ashdown, S. Poole, P. Boksa, and G. N. Luheshi The viral mimic, polyinosinic:polycytidylic acid, induces fever in rats via an interleukin-1-dependent mechanism Am J Physiol Regulatory Integrative Comp Physiol, October 1, 2004; 287(4): R759 - R766. [Abstract] [Full Text] [PDF]

				E.-M. Harre, J. Roth, U. Pehl, M. Kueth, R. Gerstberger, and T. Hubschle Molecular Biology of Thermoregulation: Selected Contribution: Role of IL-6 in LPS-induced nuclear STAT3 translocation in sensory circumventricular organs during fever in rats J Appl Physiol, June 1, 2002; 92(6): 2657 - 2666. [Abstract] [Full Text] [PDF]

				T. Oka, K. Oka, and T. Hori Mechanisms and Mediators of Psychological Stress-Induced Rise in Core Temperature Psychosom Med, May 1, 2001; 63(3): 476 - 486. [Abstract] [Full Text] [PDF]

				W. Kozak, M. J. Kluger, A. Kozak, M. Wachulec, and K. Dokladny Role of cytochrome P-450 in endogenous antipyresis Am J Physiol Regulatory Integrative Comp Physiol, August 1, 2000; 279(2): R455 - R460. [Abstract] [Full Text] [PDF]

				L. K. McCullough, Y. Takahashi, T. Le, Q. J. Pittman, and M. G. Swain Attenuated febrile response to lipopolysaccharide in rats with biliary obstruction Am J Physiol Gastrointest Liver Physiol, July 1, 2000; 279(1): G172 - G177. [Abstract] [Full Text] [PDF]

				S. A. Shore, J. H. Abraham, I. N. Schwartzman, G. G. K. Murthy, and J. D. Laporte Ventilatory responses to ozone are reduced in immature rats J Appl Physiol, June 1, 2000; 88(6): 2023 - 2030. [Abstract] [Full Text] [PDF]

				T. Mizuno, Y. Kannan, M. Tokunaga, M. Moriyama, Y. Kiso, K. Kusakabe, J. Yamate, K. Kiyomiya, and T. Sugano Role of hypothermia induced by tumor necrosis factor on apoptosis and function of inflammatory neutrophils in mice Am J Physiol Regulatory Integrative Comp Physiol, January 1, 2000; 278(1): R157 - R165. [Abstract] [Full Text] [PDF]

				L. Paul, V. Fraifeld, and J. Kaplanski Evidence supporting involvement of leukotrienes in LPS-induced hypothermia in mice Am J Physiol Regulatory Integrative Comp Physiol, January 1, 1999; 276(1): R52 - R58. [Abstract] [Full Text] [PDF]

				L. R. Leon, W. Kozak, K. Rudolph, and M. J. Kluger An antipyretic role for interleukin-10 in LPS fever in mice Am J Physiol Regulatory Integrative Comp Physiol, January 1, 1999; 276(1): R81 - R89. [Abstract] [Full Text] [PDF]

				T. Takebayashi, J. Abraham, G. G. K. Murthy, C. Lilly, I. Rodger, and S. A. Shore Role of tachykinins in airway responses to ozone in rats J Appl Physiol, August 1, 1998; 85(2): 442 - 450. [Abstract] [Full Text] [PDF]

				A. A. Romanovsky, C. T. Simons, and V. A. Kulchitsky "Biphasic" fevers often consist of more than two phases Am J Physiol Regulatory Integrative Comp Physiol, July 1, 1998; 275(1): R323 - R331. [Abstract] [Full Text] [PDF]

				A. A. Romanovsky, V. A. Kulchitsky, C. T. Simons, and N. Sugimoto Methodology of fever research: why are polyphasic fevers often thought to be biphasic? Am J Physiol Regulatory Integrative Comp Physiol, July 1, 1998; 275(1): R332 - R338. [Abstract] [Full Text] [PDF]

				A. V. Turnbull, F. J. Pitossi, J.-J. Lebrun, S. Lee, J. C. Meltzer, D. M. Nance, A. del Rey, H. O. Besedovsky, and C. Rivier Inhibition of Tumor Necrosis Factor-alpha Action within the CNS Markedly Reduces the Plasma Adrenocorticotropin Response to Peripheral Local Inflammation in Rats J. Neurosci., May 1, 1997; 17(9): 3262 - 3273. [Abstract] [Full Text] [PDF]