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Am J Physiol Regul Integr Comp Physiol 258: R1217-R1223, 1990;
0363-6119/90 $5.00
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AJP - Regulatory, Integrative and Comparative Physiology, Vol 258, Issue 5 1217-R1223, Copyright © 1990 by American Physiological Society

ARTICLES

Cell volume regulation by skate erythrocytes: role of potassium

K. G. Dickman and L. Goldstein
Department of Cell Biology, Duke University Medical Center, Durham, North Carolina 22710.

The role of K transport during cell volume regulation in response to extracellular osmolality, protein kinase C activation, and cellular Ca was examined in skate (Raja erinacea) red blood cells (RBC). Reduction of medium osmolality from 960 to 660 mosmol/kgH2O had no effect on K uptake or efflux despite a 25% increase in cell volume. Further reduction to 460 mosmol/kgH2O caused K uptake to double and K efflux to triple resulting in net K loss. Net K efflux in 460 mosmol/kgH2O medium was correlated with the presence of a regulatory volume decrease, which was sensitive to the anion transport inhibitor 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid (DIDS) and insensitive to chloride replacement. K-K exchange was absent in both isotonic and hypotonic media. Treatment with the Ca ionophore A23187 in the presence of Ca had no effect on either cell volume or K efflux in isotonic medium, indicating the absence of Ca-activated K transport. In contrast, phorbol ester treatment caused cell volume, Na content, and proton and K efflux to increase. Consistent with activation of Na-H exchange, phorbol ester effects were inhibited by dimethylamiloride. This study constitutes the first demonstration of volume-sensitive K transport in RBC from the most primitive vertebrate studied to date.


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M. W. Musch, A. B. Puffer, and L. Goldstein
Volume expansion stimulates monoubiquitination and endocytosis of surface-expressed skate anion-exchanger isoform
Am J Physiol Regulatory Integrative Comp Physiol, May 1, 2008; 294(5): R1657 - R1665.
[Abstract] [Full Text] [PDF]


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