AJP - Regulatory, Integrative and Comparative Physiology, Vol 260, Issue 6 1043-R1050, Copyright © 1991 by American Physiological Society

ARTICLES

Regulation of glomerular atrial natriuretic factor receptor subtypes by renal sympathetic nerves

H. De Leon and R. Garcia
Laboratory of Experimental Hypertension and Vasoactive Peptides, Clinical Research Institute of Montreal, Quebec, Canada.

The modulation of glomerular atrial natriuretic factor (ANF) receptors (ANF-R1 and ANF-R2) was examined in rats after unilateral (Uni-DNx), bilateral (Bil-DNx), and sham renal denervation (Sham-DNx). Glomerular ANF receptor density was higher in both left and right kidneys (1,138 +/- 73 and 952 +/- 100 fmol/mg protein, respectively) of Bil-DNx rats than in Sham-DNx animals (302 +/- 9 and 328 +/- 13 fmol/mg protein, respectively). The denervated (left) kidney from Uni-DNx rats also showed higher ANF receptor density (1,146 +/- 68 fmol/mg protein) than the contralateral untouched kidney (602 +/- 34 fmol/mg protein). Plasma ANF-(99-126) and ANF-(1-98) levels were significantly lower in the Bil-DNx group than in either Uni-DNx or Sham-DNx animals. Affinity cross-linking studies followed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and autoradiography demonstrated that both ANF-R1 and ANF-R2 receptors were upregulated in the same proportion in denervated kidneys. Guanosine 3',5'-cyclic monophosphate production by isolated glomeruli from denervated kidneys of either Bil-DNx or Uni-DNx rats was significantly higher than that of Sham-DNx controls. We conclude that renal sympathetic nerves regulate both glomerular ANF-R1 and ANF-R2-receptor subtypes.

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				H. de Leon, M.-C. Bonhomme, G. Thibault, and R. Garcia Localization of Atrial Natriuretic Factor Receptors in the Mesenteric Arterial Bed : Comparison With Angiotensin II and Endothelin Receptors Circ. Res., July 1, 1995; 77(1): 64 - 72. [Abstract] [Full Text]