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Am J Physiol Regul Integr Comp Physiol 262: R105-R111, 1992;
0363-6119/92 $5.00
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AJP - Regulatory, Integrative and Comparative Physiology, Vol 262, Issue 1 105-R111, Copyright © 1992 by American Physiological Society

ARTICLES

Carbachol induces granular cell exocytosis and serotonin release in rabbit cerebral arteries

A. M. Reynier-Rebuffel, J. Callebert, V. Dimitriadou, P. Mathiau, J. M. Launay, J. Seylaz and P. Aubineau
Laboratoire de Recherches Cerebrovasculaires, UA 641 Centre National de la Recherche Scientifique, Universite Paris VII, Paris, France.

Previously, we reported that rabbit cerebral arteries contain mast cells that frequently establish close contacts with parasympathetic-like nerve fibers. Here we have examined the possible function of this link by comparing the effects of carbachol and compound 48/80 on mast cell morphology and on the serotonin (5-HT) and histamine content of these arteries. In vivo, 2 micrograms/min of compound 48/80 or 1 micrograms/min of carbachol was infused for 30 min into one internal carotid artery of pentobarbital anesthetized rabbits, the contralateral artery being infused with vehicle. In vitro, the action of 10(-6) M carbachol was tested on isolated middle cerebral artery trees (MCAs) in the presence or absence of 10(-7) M atropine. The effects of carbachol were also tested in vitro on sympathectomized arteries. The 5-HT and histamine contents of all MCAs were measured by radioenzymatic assay, and fragments were prepared for electron microscopy. No histamine was detectable in any artery studied. The 5-HT content of arteries from control animals and those perfused with vehicle (in vivo) or incubated in the physiological solution (in vitro) was 250-300 pmol/mg protein. Both compound 48/80 and carbachol reduced this amount by approximately 50% and induced a marked degranulation of mast cells. Both secretion and degranulation were dramatically blocked in vitro by atropine. No difference in the 5-HT content was observed between intact and sympathectomized arteries under any condition. We conclude that a large proportion of rabbit cerebrovascular 5-HT is stored in mast cells and that cholinergic nerve activation could theoretically release this pool by acting on mast cell muscarinic receptors.


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I. Matsumoto, Y. Inoue, K. Tsuchiya, T. Shimada, and T. Aikawa
Degranulation of mast cells located in median eminence in response to compound 48/80 evokes adrenocortical secretion via histamine and CRF in dogs
Am J Physiol Regulatory Integrative Comp Physiol, October 1, 2004; 287(4): R969 - R980.
[Abstract] [Full Text] [PDF]


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