AJP - Regu Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Regul Integr Comp Physiol 264: R716-R719, 1993;
0363-6119/93 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Young, R. S.
Right arrow Articles by Haddad, G. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Young, R. S.
Right arrow Articles by Haddad, G. G.

AJP - Regulatory, Integrative and Comparative Physiology, Vol 264, Issue 4 716-R719, Copyright © 1993 by American Physiological Society

ARTICLES

Effect of anoxia on excitatory amino acids in brain slices of rats and turtles: in vitro microdialysis

R. S. Young, M. J. During, D. F. Donnelly, W. J. Aquila, V. L. Perry and G. G. Haddad
Department of Pediatrics, Yale University School of Medicine, New Haven, Connecticut 06510.

Using in vitro microdialysis, we tested the hypothesis that anoxia-induced release of excitatory amino acids is greater in adult rat brain than in turtle brain. Ten minutes of anoxia produced significant elevation of glutamate (from 0.39 +/- 0.03 to 0.90 +/- 0.18 microM dialysate, means +/- SE, P < 0.05), aspartate (from 0.28 +/- 0.12 to 1.20 +/- 0.49 microM, P < 0.05), glycine, and alanine in the rat brain slice. During reoxygenation, alanine and glycine returned toward baseline values, whereas aspartate and glutamate remained elevated. In contrast, prolonged anoxia (60 min) in the turtle brain slice resulted in only minimal increase in aspartate (from 0.06 +/- 0.01 to 0.09 +/- 0.02 microM, P < 0.05) and, interestingly, a decrease in glutamate (from 0.50 +/- 0.11 to 0.33 +/- 0.09 microM, P < 0.05). Levels of glycine, alanine, and taurine were unchanged. We conclude that oxygen deprivation causes marked increase in excitatory amino acids in the anoxia-sensitive rat brain slice, while oxygen deprivation for an even longer period of time in the turtle brain slice produces substantially less change. We speculate that the difference in sensitivity to anoxia between rat and turtle is at least partly attributable to the major difference in interstitial levels of excitotoxic amino acids during oxygen deprivation.


This article has been cited by other articles:


Home page
 Am. J. Physiol. Cell Physiol.Home page
J. Zhang, G. T. Gibney, P. Zhao, and Y. Xia
Neuroprotective role of delta -opioid receptors in cortical neurons
Am J Physiol Cell Physiol, June 1, 2002; 282(6): C1225 - C1234.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Q. Chen, E. Ma, K. L. Behar, T. Xu, and G. G. Haddad
Role of Trehalose Phosphate Synthase in Anoxia Tolerance and Development in Drosophila melanogaster
J. Biol. Chem., January 25, 2002; 277(5): 3274 - 3279.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
G. G. Haddad
Enhancing our understanding of the molecular responses to hypoxia in mammals using Drosophila melanogaster
J Appl Physiol, April 1, 2000; 88(4): 1481 - 1487.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
G. Hilaire and B. Duron
Maturation of the Mammalian Respiratory System
Physiol Rev, April 1, 1999; 79(2): 325 - 360.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online