AJP - Regulatory, Integrative and Comparative Physiology, Vol 265, Issue 6 1284-R1290, Copyright © 1993 by American Physiological Society

ARTICLES

Sex difference in the antidiuretic activity of vasopressin in the rat

Y. X. Wang, R. M. Edwards, P. Nambi, E. J. Stack, M. Pullen, L. Share, J. T. Crofton and D. P. Brooks
Department of Renal Pharmacology, SmithKline Beecham Pharmaceuticals, King of Prussia, Pennsylvania 19406-0939.

A possible gender difference in the antidiuretic activity of vasopressin was studied in male and female Sprague-Dawley rats. Infusion of vasopressin (3-100 pg.kg-1.min) into conscious, chronically instrumented water-loaded rats resulted in a dose-dependent antidiuresis in both male and female rats. Male rats, however, were more than three times more sensitive to vasopressin than female rats. Thus the effective doses of vasopressin (pg.kg-1.min-1) to decrease urine flow to 30 microliters.min-1.100 g-1 (18 +/- 5 in males; 58 +/- 12 in females), to increase urine osmolality to 600 mosmol/kgH2O (35 +/- 5 in males; 119 +/- 15 in females), and to decrease free water clearance to 30 microliters.min-1.100 g-1 (8 +/- 3 in males; 28 +/- 7 in females) were significantly (P < 0.05) lower in males. Furthermore, in vitro studies in papillary collecting duct cells demonstrated a significantly higher density of vasopressin V2 receptors and a greater ability of vasopressin to stimulate adenosine 3',5'-cyclic monophosphate (cAMP) accumulation in males than in females. Vasopressin V2-receptor density (maximum binding) was 359 +/- 47 and 238 +/- 22 fmol/mg in male and female rats, respectively (P < 0.05). There was no difference in apparent dissociation constants (Kd). Vasopressin resulted in a dose-dependent increase in cAMP accumulation in papillary collecting duct cells, and at the highest concentration of vasopressin used (10(-8) M) cAMP increased from 44 +/- 10 to 182 +/- 51 fmol/micrograms protein in males and from 30 +/- 4 to 91 +/- 18 fmol/micrograms protein in females (P < 0.05). (ABSTRACT TRUNCATED AT 250 WORDS)

This article has been cited by other articles:

				J. Perucca, N. Bouby, P. Valeix, and L. Bankir Sex difference in urine concentration across differing ages, sodium intake, and level of kidney disease Am J Physiol Regulatory Integrative Comp Physiol, February 1, 2007; 292(2): R700 - R705. [Abstract] [Full Text] [PDF]

				S. J. Somponpun, A. K. Johnson, T. Beltz, and C. D. Sladek Estrogen receptor-{alpha} expression in osmosensitive elements of the lamina terminalis: regulation by hypertonicity Am J Physiol Regulatory Integrative Comp Physiol, September 1, 2004; 287(3): R661 - R669. [Abstract] [Full Text] [PDF]

				D. S. Knee, A. K. Sato, C. F. T. Uyehara, and J. R. Claybaugh Prenatal exposure to ethanol causes partial diabetes insipidus in adult rats Am J Physiol Regulatory Integrative Comp Physiol, August 1, 2004; 287(2): R277 - R283. [Abstract] [Full Text] [PDF]

				J. R. Claybaugh, A. K. Sato, L. K. Crosswhite, and L. H. Hassell Effects of time of day, gender, and menstrual cycle phase on the human response to a water load Am J Physiol Regulatory Integrative Comp Physiol, September 1, 2000; 279(3): R966 - R973. [Abstract] [Full Text] [PDF]

				D. P. Brooks, P. Nambi, N. J. Laping, B. A. Olson, M. Pullen, and C. E. Wade Renal vasopressin receptor expression and function in rats following spaceflight J Appl Physiol, April 1, 2000; 88(4): 1316 - 1320. [Abstract] [Full Text] [PDF]

				E. Brand, L. Bankir, P.-F. Plouin, and F. Soubrier Glucagon Receptor Gene Mutation (Gly40Ser) in Human Essential Hypertension : The PEGASE Study Hypertension, July 1, 1999; 34(1): 15 - 17. [Abstract] [Full Text] [PDF]