AJP - Regulatory, Integrative and Comparative Physiology, Vol 266, Issue 2 419-R425, Copyright © 1994 by American Physiological Society

ARTICLES

Whole body and splanchnic leucine, phenylalanine, and glucose kinetics during endotoxemia in humans

Y. Fong, D. E. Matthews, W. He, M. A. Marano, L. L. Moldawer and S. F. Lowry
Department of Surgery, New York Hospital-Cornell Medical Center, New York 10021.

To examine the whole body and splanchnic tissue substrate handling during endotoxemia, an intravenous bolus of endotoxin was given to six healthy volunteers during primed, continuous infusions of [1-13C]leucine, [ring-2H5]phenylalanine, and [6,6-2H2]glucose. Whole body protein breakdown, based on whole body Leu and Phe appearance rates (Ra), increased in response to endotoxin given at time 0 (RaLeu 77 +/- 2 mol.kg-1 x h-1, t = 0 h; 88 +/- 6, t = 4 h; P < 0.05) (RaPhe 39 +/- 2 mol.kg-1 x h-1, t = 0; 46 +/- 3, t = 4 h; P < 0.05). Splanchnic amino acid balance (Bal) increased (BalLeu 7 +/- 4 mol.kg-1 x h-1, t = 0; 21 +/- 5, t = 2 h; P < 0.05) (BalPhe 3 +/- 2 mol.kg-1 x h-1, t = 0; 16 +/- 4, t = 2 h; P < 0.05) and can be accounted for by increased splanchnic uptake (Rd) of Phe and Leu (RdLeu 21 +/- 3 mol.kg-1 x h -1, t = 0; 37 +/- 7, t = 120 min; P < 0.05) (RdPhe 10 +/- 3 mol.kg-1 x h-1, t = 0; 24 +/- 5, t = 120 min; P < 0.05). Splanchnic conversion of Leu to ketoisocaproate increased with endotoxin administration (0.7 +/- 0.6 mol.kg-1 x h-1, t = 0; 8 +/- 3, t = 360 min; P < 0.05).(ABSTRACT TRUNCATED AT 250 WORDS)

This article has been cited by other articles:

				M. J. Toth, B. C. Cooper, R. E. Pratley, A. Mari, D. E. Matthews, and P. R. Casson Effect of ovarian suppression with gonadotropin-releasing hormone agonist on glucose disposal and insulin secretion Am J Physiol Endocrinol Metab, June 1, 2008; 294(6): E1035 - E1045. [Abstract] [Full Text] [PDF]

				S. Mercier, D. Breuille, L. Mosoni, C. Obled, and P. Patureau Mirand Chronic Inflammation Alters Protein Metabolism in Several Organs of Adult Rats J. Nutr., July 1, 2002; 132(7): 1921 - 1928. [Abstract] [Full Text] [PDF]

				M. J. Toth, C. K. Sites, W. T. Cefalu, D. E. Matthews, and E. T. Poehlman Determinants of insulin-stimulated glucose disposal in middle-aged, premenopausal women Am J Physiol Endocrinol Metab, July 1, 2001; 281(1): E113 - E121. [Abstract] [Full Text] [PDF]

				M. J. Manary, K. E. Yarasheski, C. A. Hart, and R. L. Broadhead Plasma Urea Appearance Rate Is Lower When Children with Kwashiorkor and Infection Are Fed Egg White-Tryptophan Rather than Milk Protein J. Nutr., January 1, 2000; 130(2): 183 - 188. [Abstract] [Full Text]

				F. Skopec, C. Andrýs, and M. Pecka Leucine metabolism in TNF-alpha - and endotoxin-treated rats: contribution of hepatic tissue Am J Physiol Endocrinol Metab, December 1, 1997; 273(6): E1052 - E1058. [Abstract] [Full Text]

				M. M. Hallemeesch, P. B. Soeters, and N. E. P. Deutz Renal arginine and protein synthesis are increased during early endotoxemia in mice Am J Physiol Renal Physiol, February 1, 2002; 282(2): F316 - F323. [Abstract] [Full Text] [PDF]