| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
AJP - Regulatory, Integrative and Comparative Physiology, Vol 267, Issue 4 1020-R1025, Copyright © 1994 by American Physiological Society
ARTICLES |
G. Williams, T. Brown, L. Becker, M. Prager and B. P. Giroir
Department of Pediatrics, University of Texas Southwestern Medical Center, Dallas 75235.
Nitric oxide (NO) is an important mediator of diverse physiological and pathological responses. To determine whether NO production can be induced in skeletal muscle, we stimulated C2C12 mouse skeletal muscle myocytes with putative inducers of nitric oxide synthase (NOS). Neither lipopolysaccharide (LPS), interleukin-1 alpha (IL-1), tumor necrosis factor-alpha (TNF), nor interferon-gamma (IFN) was able to stimulate nitrite production by C2C12 cells when administered alone. However, combinations of IFN with either TNF or IL-1 resulted in significant nitrite production; simultaneous stimulation of cells with all three cytokines resulted in significantly increased nitrite production compared with any combination of two cytokines. Northern analysis of RNA obtained from stimulated C2C12 cells revealed induction of a single mRNA band that precisely coincided with the mRNA band of mouse macrophage-inducible NOS (iNOS). Reverse transcriptase-polymerase chain reaction (RT-PCR) analysis followed by sequencing of the 5' 765 bases of the skeletal muscle iNOS cDNA demonstrated exact homology with mouse macrophage iNOS. These findings indicate that combinations of cytokines stimulate NO production in skeletal muscle cells via induction of the macrophage-type iNOS gene.
This article has been cited by other articles:
|
|
 |
|
  T. Vassilakopoulos and S. N. A. Hussain Ventilatory muscle activation and inflammation: cytokines, reactive oxygen species, and nitric oxide J Appl Physiol, April 1, 2007; 102(4): 1687 - 1695. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Vassilakopoulos, K. Govindaraju, D. Parthenis, D. H. Eidelman, Y. Watanabe, and S. N. A. Hussain Nitric oxide production in the ventilatory muscles in response to acute resistive loading Am J Physiol Lung Cell Mol Physiol, April 1, 2007; 292(4): L1013 - L1022. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Vassilakopoulos, G. Deckman, M. Kebbewar, G. Rallis, R. Harfouche, and S. N. A. Hussain Regulation of nitric oxide production in limb and ventilatory muscles during chronic exercise training Am J Physiol Lung Cell Mol Physiol, March 1, 2003; 284(3): L452 - L457. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Fernandez-Celemin, N. Pasko, V. Blomart, and J.-P. Thissen Inhibition of muscle insulin-like growth factor I expression by tumor necrosis factor-alpha Am J Physiol Endocrinol Metab, December 1, 2002; 283(6): E1279 - E1290. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. C. J. Langen, A. M. W. J. Schols, M. C. J. M. Kelders, J. L. J. van der Velden, E. F. M. Wouters, and Y. M. W. Janssen-Heininger Tumor necrosis factor-alpha inhibits myogenesis through redox-dependent and -independent pathways Am J Physiol Cell Physiol, September 1, 2002; 283(3): C714 - C721. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. S. Stamler and G. Meissner Physiology of Nitric Oxide in Skeletal Muscle Physiol Rev, January 1, 2001; 81(1): 209 - 237. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Li, M. R. Moody, D. Engel, S. Walker, F. J. Clubb Jr, N. Sivasubramanian, D. L. Mann, and M. B. Reid Cardiac-Specific Overexpression of Tumor Necrosis Factor-{alpha} Causes Oxidative Stress and Contractile Dysfunction in Mouse Diaphragm Circulation, October 3, 2000; 102(14): 1690 - 1696. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Zhang, G. Pilon, A. Marette, and V. E. Baracos Cytokines and endotoxin induce cytokine receptors in skeletal muscle Am J Physiol Endocrinol Metab, July 1, 2000; 279(1): E196 - E205. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Baron, D. Galimberti, L. Meda, E. Prat, E. Scarpini, G. Conti, M. Moggio, A. Prelle, and G. Scarlato Synergistic effect of {beta}-amyloid protein and interferon gamma on nitric oxide production by C2C12 muscle cells Brain, February 1, 2000; 123(2): 374 - 379. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Fujii, Y. Guo, and S. N. A. Hussain Regulation of nitric oxide production in response to skeletal muscle activation J Appl Physiol, December 1, 1998; 85(6): 2330 - 2336. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. El-Dwairi, A. Comtois, Y. Guo, and S. N. A. Hussain Endotoxin-induced skeletal muscle contractile dysfunction: contribution of nitric oxide synthases Am J Physiol Cell Physiol, March 1, 1998; 274(3): C770 - C779. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R.-S. Zhang, P. H. Guth, O. U. Scremin, R. Singh, S. Pervin, and G. Chaudhuri Regulation of endometrial blood flow in ovariectomized rats: assessment of the role of nitric oxide Am J Physiol Heart Circ Physiol, October 1, 1997; 273(4): H2009 - H2017. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. N. A. Hussain, A. Giaid, Q. E. Dawiri, D. Sakkal, R. Hattori, and Y. Guo Expression of Nitric Oxide Synthases and GTP Cyclohydrolase I in the Ventilatory and Limb Muscles During Endotoxemia Am. J. Respir. Cell Mol. Biol., August 1, 1997; 17(2): 173 - 180. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
