AJP - Regulatory, Integrative and Comparative Physiology, Vol 270, Issue 3 578-R584, Copyright © 1996 by American Physiological Society

ARTICLES

Control of acid secretion in cultured gas gland cells of the European eel Anguilla anguilla

B. Pelster and L. Pott
Institut fur Physiologie, Ruhr-Universitat Bochum, Germany.

Single cells and cell clusters isolated from the swimbladder epithelium of the European eel Anguilla anguilla attached to collagen S-coated petri dishes and proliferated in a modified Dulbecco's modified Eagle's medium, supplemented with 0.5% fetal calf serum. At a temperature of 20-22 degrees C, the growing colonies reached confluence typically within 6-8 days. Activities of glycolytic and pentose phosphate shunt enzymes remained stable or increased only slightly during the first 10 days of primary culture. Incubated in a defined medium providing glucose as a fuel, gas gland cells in primary culture produced and released lactic acid. The rate of acid secretion of cultured gas gland cells measured with a cytosensor microphysiometer was not influenced by cholinergic stimulation. Similarly, the Ca2+ ionophore A-23187 had no effect. Adrenergic stimulation with epinephrine or the beta-agonist isoproterenol also did not increase the rate of acid secretion, indicating that in gas gland cells the metabolic activity cannot be stimulated via beta-adrenergic stimulation followed by an increase in adenosine 3',5'-cyclic monophosphate (cAMP). Artificially increasing the intracellular concentration of cAMP by incubation with forskolin or the cAMP analogue 8-(4-chlorophenylthio)-cAMP even resulted in a marked reduction in the rate of acid secretion. The results demonstrate that primary cell culture provides a useful means for the analysis of metabolic control and of ion transfer processes in swimbladder gas gland cells.

This article has been cited by other articles:

				E. Sotz, H. Niederstatter, and B. Pelster Determinants of intracellular pH in gas gland cells of the swimbladder of the European eel Anguilla anguilla J. Exp. Biol., April 15, 2002; 205(8): 1069 - 1075. [Abstract] [Full Text] [PDF]

				B. Pelster and H. Niederstatter pH-dependent proton secretion in cultured swim bladder gas gland cells Am J Physiol Regulatory Integrative Comp Physiol, November 1, 1997; 273(5): R1719 - R1725. [Abstract] [Full Text] [PDF]