AJP - Regu AJP: Gastrointestinal and Liver Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Regul Integr Comp Physiol 270: R766-R776, 1996;
0363-6119/96 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Dyck, D. J.
Right arrow Articles by Spriet, L. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Dyck, D. J.
Right arrow Articles by Spriet, L. L.

AJP - Regulatory, Integrative and Comparative Physiology, Vol 270, Issue 4 766-R776, Copyright © 1996 by American Physiological Society

ARTICLES

Effect of high FFA on glycogenolysis in oxidative rat hindlimb muscles during twitch stimulation

D. J. Dyck, S. J. Peters, P. S. Wendling and L. L. Spriet
School of Human Biology, University of Guelph, Ontario, Canada.

The effect of elevated free fatty acids (FFA) on carbohydrate (CHO) utilization in the oxidative muscles of the isolated hindlimb was determined using twitch contraction paradigms evoking a wide range of O2 uptakes and glycogenolysis. The hindlimb was perfused with either 0 or 1.8 mM FFA for 10 min at rest and then subjected to 20 min of stimulation at 0.4, 0.7, 1, 2, 3, or 4 Hz. Soleus (Sol), plantaris (Pl), and red gastrocnemius (RG) were sampled after rest perfusion or stimulation. FFA had little effect on glycogenolysis during stimulation, although glycogen sparing occurred with one of the lesser intensity protocols in each muscle (Sol, 0.4 Hz; RG, 0.7 Hz; Pl, 1 Hz). Muscle citrate and acetyl-CoA were elevated in Sol during several stimulation protocols with high FFA, but this effect was inconsistent in Pl and RG. The sparing of glycogen, when it did occur, was generally unrelated to increases in either citrate or acetyl-CoA content. Furthermore, protocols in which citrate or acetyl-CoA were elevated in the presence of elevated FFA did not demonstrate glycogen sparing. Hindlimb lactate efflux at rest was reduced with FFA but unaffected during stimulation. Glucose uptake was unaffected by FFA at rest and during stimulation protocols, except 3 Hz. The present study does not support the classically proposed roles of citrate and acetyl-CoA in the FFA-induced downregulation of CHO utilization in electrically stimulated rat skeletal muscle.


This article has been cited by other articles:


Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
T. Stellingwerff, M. J. Watt, G. J. F. Heigenhauser, and L. L. Spriet
Effects of reduced free fatty acid availability on skeletal muscle PDH activation during aerobic exercise
Am J Physiol Endocrinol Metab, March 1, 2003; 284(3): E589 - E596.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
D. J. Dyck, G. Steinberg, and A. Bonen
Insulin increases FA uptake and esterification but reduces lipid utilization in isolated contracting muscle
Am J Physiol Endocrinol Metab, September 1, 2001; 281(3): E600 - E607.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online