AJP - Regulatory, Integrative and Comparative Physiology, Vol 271, Issue 2 352-R360, Copyright © 1996 by American Physiological Society

ARTICLES

Bradykinin B2 receptor antagonist increases chloride and water absorption in rat medullary collecting duct

H. Mukai, W. R. Fitzgibbon, G. Bozeman, H. S. Margolius and D. W. Ploth
Department of Medicine, Medical University of South Carolina, Charleston 29425, USA.

This study tested the hypothesis that intrarenal kinins play a regulatory role in electrolyte excretion by altering Cl- absorption in the collecting duct. We measured Cl- and insulin concentrations in tubular fluid samples obtained from medullary collecting ducts (MCD) of Dahl/Rapp salt-resistant (SR/ Jr) rats by microcatheterization of ducts of Bellini before and after treatment with the bradykinin receptor antagonist HOE-140. Tubular fluid was obtained from paired terminal inner medullary (t-IMCD) and outer medullary (OMCD) collecting duct sites of the left kidney. HOE-140 (n = 7) or vehicle (n = 5) was infused intravenously, and the collections were repeated. HOE-140 did not alter glomerular filtration rate but decreased urine flow rate (P < 0.05) and absolute and fractional Cl- excretion (P < 0.01). HOE-140 did not alter the fraction of filtered Cl- delivered (FDCl) to the OMCD but decreased FDCl to the t-IMCD from 2.3 +/- 0.3 to 1.3 +/- 0.3% (P < 0.05). The fraction of filtered Cl- absorbed per millimeter between the collection sites was increased from 0.2 +/- 0.1 to 0.6 +/- 0.1% (P < 0.05). Fractional absorption of water along the MCD was also increased (P < 0.05). No changes in excretory function or tubular Cl- or water absorption were observed in vehicle-treated rats. These studies show that kinin B2 receptor blockade enhances Cl- and water absorption in the MCD, a finding that supports a role of renal kinins in the regulation of NaCl and water excretion.

This article has been cited by other articles:

				A. Duka, I. Duka, G. Gao, S. Shenouda, I. Gavras, and H. Gavras Role of bradykinin B1 and B2 receptors in normal blood pressure regulation Am J Physiol Endocrinol Metab, August 1, 2006; 291(2): E268 - E274. [Abstract] [Full Text] [PDF]

				Y. V. Mukhin, E. A. Garnovsky, M. E. Ullian, and M. N. Garnovskaya Bradykinin B2 Receptor Activates Extracellular Signal-Regulated Protein Kinase in mIMCD-3 Cells via Epidermal Growth Factor Receptor Transactivation J. Pharmacol. Exp. Ther., March 1, 2003; 304(3): 968 - 977. [Abstract] [Full Text] [PDF]

				G. Liu, L. Kleine, R. Nasrallah, and R. L. Hebert Bradykinin inhibits ceramide production and activates phospholipase D in rabbit cortical collecting duct cells Am J Physiol Renal Physiol, April 1, 1999; 276(4): F589 - F598. [Abstract] [Full Text] [PDF]

				Y. V. Mukhin, T. Vlasova, A. A. Jaffa, G. Collinsworth, J. L. Bell, B. G. Tholanikunnel, T. Pettus, W. Fitzgibbon, D. W. Ploth, J. R. Raymond, et al. Bradykinin B2 Receptors Activate Na+/H+ Exchange in mIMCD-3 Cells via Janus Kinase 2 and Ca2+/Calmodulin J. Biol. Chem., May 11, 2001; 276(20): 17339 - 17346. [Abstract] [Full Text] [PDF]