AJP - Regu Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Regul Integr Comp Physiol 271: R696-R703, 1996;
0363-6119/96 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Reiser, K.
Right arrow Articles by McDonald, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Reiser, K.
Right arrow Articles by McDonald, R.

AJP - Regulatory, Integrative and Comparative Physiology, Vol 271, Issue 3 696-R703, Copyright © 1996 by American Physiological Society

ARTICLES

Effects of elevated circulating IGF-1 on the extracellular matrix in "high-growth" C57BL/6J mice

K. Reiser, P. Summers, J. F. Medrano, R. Rucker, J. Last and R. McDonald
Department of Internal Medicine, School of Medicine, University of California, Davis 95616, USA.

Collagen biosynthesis was analyzed in C57BL/6J mice homozygous for the high-growth locus. Plasma levels of insulin-like growth factor-1 (IGF-1) were significantly elevated in high-growth mice at all ages studied (3 wk-6 mo); IGF-binding proteins were also elevated. Skin biopsies were obtained from mice aged 3, 6, and 9 wk under halothane anesthesia. Mice were killed at 6 mo of age. Collagen, expressed per weight of tissue, was significantly increased in all tissues from high-growth mice, as was collagen cross-linking, expressed as moles of cross-link per mole of collagen. Expression of types I and III collagen, lysyl oxidase, and lysyl hydroxylase was increased in all tissues analyzed. There was a preferential increase in type III expression relative to type I expression. Rate and extent of accumulation of collagen in granulation tissue were measured in polyvinyl alcohol sponges implanted subcutaneously; collagen accumulation was significantly greater in the high-growth mice. These results suggest that 1) elevated circulating IGF-1 may increase collagen deposition both in normal tissue as well as in granulation tissue by increasing collagen gene expression, 2) IGF-1 may increase collagen cross-linking by stimulating expression of lysyl oxidase, and 3) the preferential increase in dihydroxylated cross-links observed in high-growth mice may be due to the stimulation of lysyl hydroxylase expression by IGF-1. In summary, elevated levels of IGF-1 appear to affect collagen both quantitatively and qualitatively, primarily through their effects on gene expression of collagen and of those enzymes responsible for posttranslational modifications of collagen.


This article has been cited by other articles:


Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
J. Behmoaras, M. Osborne-Pellegrin, D. Gauguier, and M.-P. Jacob
Characteristics of the aortic elastic network and related phenotypes in seven inbred rat strains
Am J Physiol Heart Circ Physiol, February 1, 2005; 288(2): H769 - H777.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. GenomicsHome page
P. M. CORVA and J. F. MEDRANO
Diet effects on weight gain and body composition in high growth (hg/hg) mice
Physiol Genomics, June 29, 2000; 3(1): 17 - 23.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. H. Shin, C. Ji, S. Casinghino, T. L. McCarthy, and M. Centrella
Parathyroid Hormone-related Protein Enhances Insulin-like Growth Factor-I Expression by Fetal Rat Dermal Fibroblasts
J. Biol. Chem., September 19, 1997; 272(38): 23498 - 23502.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online