AJP - Regulatory, Integrative and Comparative Physiology, Vol 271, Issue 6 1729-R1738, Copyright © 1996 by American Physiological Society

ARTICLES

Beta 3-adrenoceptor in guinea pig brown and white adipocytes: low expression and lack of function

C. Atgie, G. Tavernier, F. D'Allaire, T. Bengtsson, L. Marti, C. Carpene, M. Lafontan, L. J. Bukowiecki and D. Langin
Department of Physiology, Medical School, Laval University, Quebec, Canada.

In the guinea pig, cold acclimation induced a conversion of unilocular to multilocular adipocytes in interscapular (IS) and retroperitoneal (RP) fat depots but not in the epididymal (EP) fat pad. The conversion was associated with an increase in mitochondriogenesis and the appearance of the uncoupling protein. The maximal lipolytic responses to norepinephrine and dibutyryl adenosine 3',5'-cyclic monophosphate were decreased in IS cells, unchanged in RP cells, and increased in EP cells, suggesting a site-specific regulation of lipolysis at the postreceptor level. beta 3-Adrenergic agonists were not lipolytic regardless of the depot and the thermal environment of the animal. These agents did not inhibit glucose transport and lipogenesis, as was previously reported for rodents. Cloning and sequencing of the guinea pig beta 3-adrenoceptor gene revealed a slightly higher amino acid sequence similarity with the human than with the rodent beta 3-adrenoceptors. beta 3-Adrenoceptor transcripts were present at a very low level in guinea pig adipocytes, and mRNA levels did not increase to a significant extent after cold acclimation. The guinea pig thus differs from rodents by an absence of beta 3-adrenergic effects and by low beta 3-adrenoceptor expression in brown and white adipose tissues.

This article has been cited by other articles:

				K. L. Kind, C. T. Roberts, A. I. Sohlstrom, A. Katsman, P. M. Clifton, J. S. Robinson, and J. A. Owens Chronic maternal feed restriction impairs growth but increases adiposity of the fetal guinea pig Am J Physiol Regulatory Integrative Comp Physiol, January 1, 2005; 288(1): R119 - R126. [Abstract] [Full Text] [PDF]

				B. CANNON and J. NEDERGAARD Brown Adipose Tissue: Function and Physiological Significance Physiol Rev, January 1, 2004; 84(1): 277 - 359. [Abstract] [Full Text] [PDF]

				J. G. Nickerson, S. G. Dugan, G. Drouin, S. F. Perry, and T. W. Moon Activity of the unique {beta}-adrenergic Na+/H+ exchanger in trout erythrocytes is controlled by a novel {beta}3-AR subtype Am J Physiol Regulatory Integrative Comp Physiol, September 1, 2003; 285(3): R526 - R535. [Abstract] [Full Text] [PDF]

				M. R. Candelore, L. Deng, L. Tota, X.-M. Guan, A. Amend, Y. Liu, R. Newbold, M. A. Cascieri, and A. E. Weber Potent and Selective Human beta 3-Adrenergic Receptor Antagonists J. Pharmacol. Exp. Ther., August 1, 1999; 290(2): 649 - 655. [Abstract] [Full Text]

				J. Zhao, B. Cannon, and J. Nedergaard Thermogenesis is beta 3- but not beta 1-adrenergically mediated in rat brown fat cells, even after cold acclimation Am J Physiol Regulatory Integrative Comp Physiol, December 1, 1998; 275(6): R2002 - R2011. [Abstract] [Full Text] [PDF]

				X. Liu, F. Perusse, and L. J. Bukowiecki Mechanisms of the antidiabetic effects of the beta 3-adrenergic agonist CL-316243 in obese Zucker-ZDF rats Am J Physiol Regulatory Integrative Comp Physiol, May 1, 1998; 274(5): R1212 - R1219. [Abstract] [Full Text] [PDF]

				C. Atgie, F. D'Allaire, and L. J. Bukowiecki Role of beta 1- and beta 3-adrenoceptors in the regulation of lipolysis and thermogenesis in rat brown adipocytes Am J Physiol Cell Physiol, October 1, 1997; 273(4): C1136 - C1142. [Abstract] [Full Text] [PDF]