AJP - Regulatory, Integrative and Comparative Physiology, Vol 272, Issue 3 1007-R1011, Copyright © 1997 by American Physiological Society

ARTICLES

Leptin-induced decrease in food intake is not associated with changes in gastric emptying in lean mice

M. D. Barrachina, V. Martinez, J. Y. Wei and Y. Tache
Center for Ulcer Research and Education, West Los Angeles Veteran's Affairs Medical Center, California 90073, USA.

Chronic treatment with leptin regulates body weight and energy balance and reduces food intake in obese and lean mice. In 18- to 20-h fasted lean mice (C57BL/6, +/+), we examined the acute effect of a single intraperitoneal injection of recombinant mouse leptin (0.12 mg/kg) on food intake and gastric emptying. Leptin reduced food intake, with a peak inhibition at the 5th h postinjection (69 +/- 12%/h), although there was no change in food consumption at the 1st h. Leptin did not alter the 4-h rate of gastric emptying of a solid nutrient meal (free access to Purina chow for either 1-, 2-, or 4-h period). In normal Sprague-Dawley rats fasted for 18-20 h, a single intraperitoneal injection of recombinant mouse leptin (0.2 or 1.2 mg/kg) did not modify the 7-h cumulative or hourly food intake. These results show that a single intraperitoneal injection of recombinant mouse leptin reduces food intake within 5 h while not influencing gastric emptying of ingested food in lean mice. Sprague-Dawley rats are unresponsive to the food intake-reducing effect of a single intraperitoneal injection of mouse leptin at a dose 10-fold higher than that shown to be effective in mice within the first 4-7 h postinjection.

This article has been cited by other articles:

				V. Martinez, L. Wang, J. E. Rivier, W. Vale, and Y. Tache Differential Actions of Peripheral Corticotropin-Releasing Factor (CRF), Urocortin II, and Urocortin III on Gastric Emptying and Colonic Transit in Mice: Role of CRF Receptor Subtypes 1 and 2 J. Pharmacol. Exp. Ther., May 1, 2002; 301(2): 611 - 617. [Abstract] [Full Text] [PDF]

				D. Matzinger, J.-P. Gutzwiller, J. Drewe, A. Orban, R. Engel, M. D'Amato, L. Rovati, and C. Beglinger Inhibition of food intake in response to intestinal lipid is mediated by cholecystokinin in humans Am J Physiol Regulatory Integrative Comp Physiol, December 1, 1999; 277(6): R1718 - R1724. [Abstract] [Full Text] [PDF]

				M. Romon, P. Lebel, C. Velly, N. Marecaux, J. C. Fruchart, and J. Dallongeville Leptin response to carbohydrate or fat meal and association with subsequent satiety and energy intake Am J Physiol Endocrinol Metab, November 1, 1999; 277(5): E855 - E861. [Abstract] [Full Text] [PDF]

				M. D. Barrachina, V. Martinez, L. Wang, J. Y. Wei, and Y. Tache Synergistic interaction between leptin and cholecystokinin to reduce short-term food intake in lean mice PNAS, September 16, 1997; 94(19): 10455 - 10460. [Abstract] [Full Text] [PDF]