| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
AJP - Regulatory, Integrative and Comparative Physiology, Vol 272, Issue 5 1552-R1561, Copyright © 1997 by American Physiological Society
ARTICLES |
J. J. McCarthy, A. M. Fox, G. L. Tsika, L. Gao and R. W. Tsika
University of Illinois, Urbana-Champaign 61801, USA.
Non-weight-bearing (NWB) activity [space flight and hindlimb suspension (HS)] results in the loss of soleus muscle mass, a slow-to-fast fiber-type conversion, and decreased beta-myosin heavy chain (beta-MHC) protein and mRNA expression. To identify beta-MHC promoter sequences required for decreased beta-MHC expression in response to HS, we have modified an existing noninvasive hindlimb unweighting model to accommodate the use of (transgenic) mice. After 2 wk of HS, body and muscle (soleus > gastrocnemius > plantaris) weights were decreased as was the proportion of histochemically classified type I fibers in HS soleus muscle. Northern blot analysis revealed decreases in endogenous mRNA representing beta-MHC, slow myosin light chain 1 and 2, and cardiac/slow troponin C, whereas those representing skeletal troponin C, muscle creatine kinase, and glyceraldehyde-3-phosphate dehydrogenase increased. Protein extracts prepared from HS soleus (SS) muscle of mice harboring transgenes comprised of 5.6 or 0.6 kilobase of wild type (wt) mouse beta-MHC promoter (beta 5.6 wt, beta 0.6wt) and those carrying the simultaneous mutation (mut) of the MCAT, C-rich, and beta e3 subregions (beta 5.6mut3, beta 0.6mut3) revealed decreases in chloramphenicol acetyltransferase (CAT) specific activity relative to respective controls. Decreased CAT mRNA was observed for transgene beta 5.6mut3, line 85. Two weeks of the simultaneous imposition of mechanical overload (synergist ablation) and HS (MOV/HS) countermanded the loss in absolute and normalized SS weight but did not decrease beta 0.6wt transgene expression. These transgenic results demonstrate that regulatory sequences within a 600-base pair beta-MHC promoter are sufficient to direct decreased transcription of beta-MHC transgenes after 2 wk of HS.
This article has been cited by other articles:
|
|
 |
|
  E. E. Dupont-Versteegden, R. Nagarajan, M. L. Beggs, E. D. Bearden, P. M. Simpson, and C. A. Peterson Identification of cold-shock protein RBM3 as a possible regulator of skeletal muscle size through expression profiling Am J Physiol Regulatory Integrative Comp Physiol, October 1, 2008; 295(4): R1263 - R1273. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. L. Nichols, N. Zhang, and X. Wen Proteomics and genomics of microgravity Physiol Genomics, September 14, 2006; 26(3): 163 - 171. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. M. Giger, F. Haddad, A. X. Qin, M. Zeng, and K. M. Baldwin Effect of unloading on type I myosin heavy chain gene regulation in rat soleus muscle J Appl Physiol, April 1, 2005; 98(4): 1185 - 1194. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Dapp, S. Schmutz, H. Hoppeler, and M. Fluck Transcriptional reprogramming and ultrastructure during atrophy and recovery of mouse soleus muscle Physiol Genomics, December 15, 2004; 20(1): 97 - 107. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. E. Stelzer and J. J. Widrick Effect of hindlimb suspension on the functional properties of slow and fast soleus fibers from three strains of mice J Appl Physiol, December 1, 2003; 95(6): 2425 - 2433. [Abstract] [Full Text]
|
|
|
|
|
|
B. C. Harrison, D. L. Allen, B. Girten, L. S. Stodieck, P. J. Kostenuik, T. A. Bateman, S. Morony, D. Lacey, and L. A. Leinwand Skeletal muscle adaptations to microgravity exposure in the mouse J Appl Physiol, December 1, 2003; 95(6): 2462 - 2470. [Abstract] [Full Text]
|
|
|
|
|
|
L. Bey, N. Akunuri, P. Zhao, E. P. Hoffman, D. G. Hamilton, and M. T. Hamilton Patterns of global gene expression in rat skeletal muscle during unloading and low-intensity ambulatory activity Physiol Genomics, April 16, 2003; 13(2): 157 - 167. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. A. Huey, F. Haddad, A. X. Qin, and K. M. Baldwin Transcriptional regulation of the type I myosin heavy chain gene in denervated rat soleus Am J Physiol Cell Physiol, March 1, 2003; 284(3): C738 - C748. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. W. Tsika, J. McCarthy, N. Karasseva, Y. Ou, and G. L. Tsika Divergence in species and regulatory role of beta -myosin heavy chain proximal promoter muscle-CAT elements Am J Physiol Cell Physiol, December 1, 2002; 283(6): C1761 - C1775. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. R. Morey-Holton and R. K. Globus Hindlimb unloading rodent model: technical aspects J Appl Physiol, April 1, 2002; 92(4): 1367 - 1377. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. M. Baldwin and F. Haddad Plasticity in Skeletal, Cardiac, and Smooth Muscle: Invited Review: Effects of different activity and inactivity paradigms on myosin heavy chain gene expression in striated muscle J Appl Physiol, January 1, 2001; 90(1): 345 - 357. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. H. Fitts, D. R. Riley, and J. J. Widrick Physiology of a Microgravity Environment Invited Review: Microgravity and skeletal muscle J Appl Physiol, August 1, 2000; 89(2): 823 - 839. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Giger, F. Haddad, A. X. Qin, and K. M. Baldwin In vivo regulation of the beta -myosin heavy chain gene in soleus muscle of suspended and weight-bearing rats Am J Physiol Cell Physiol, June 1, 2000; 278(6): C1153 - C1161. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. A. Lowe, H. Degens, K. D. Chen, and S. E. Alway Glyceraldehyde-3-Phosphate Dehydrogenase Varies With Age in Glycolytic Muscles of Rats J. Gerontol. A Biol. Sci. Med. Sci., March 1, 2000; 55(3): 160B - 164. [Abstract] [Full Text]
|
|
|
|
|
|
J. A. Carson and L. Wei Integrin signaling's potential for mediating gene expression in hypertrophying skeletal muscle J Appl Physiol, January 1, 2000; 88(1): 337 - 343. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. R. Vyas, J. J. McCarthy, and R. W. Tsika Nuclear Protein Binding at the beta -Myosin Heavy Chain A/T-rich Element Is Enriched following Increased Skeletal Muscle Activity J. Biol. Chem., October 22, 1999; 274(43): 30832 - 30842. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. J. McCarthy, D. R. Vyas, G. L. Tsika, and R. W. Tsika Segregated Regulatory Elements Direct beta -Myosin Heavy Chain Expression in Response to Altered Muscle Activity J. Biol. Chem., May 14, 1999; 274(20): 14270 - 14279. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. G. Peters, H. Mitchell-Felton, and S. C. Kandarian Unloading induces transcriptional activation of the sarco(endo)plasmic reticulum Ca2+-ATPase 1 gene in muscle Am J Physiol Cell Physiol, May 1, 1999; 276(5): C1218 - C1225. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Cros, J. Muller, S. Bouju, G. Pietu, C. Jacquet, J. J. Leger, J.-F. Marini, and C. A. Dechesne Upregulation of M-creatine kinase and glyceraldehyde3-phosphate dehydrogenase: two markers of muscle disuse Am J Physiol Regulatory Integrative Comp Physiol, February 1, 1999; 276(2): R308 - R316. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Kell, H. Pierce, and S. J. Swoap PGAM-M expression is regulated pretranslationally in hindlimb muscles and under altered loading conditions J Appl Physiol, January 1, 1999; 86(1): 236 - 242. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. S. Criswell, F. W. Booth, F. DeMayo, R. J. Schwartz, S. E. Gordon, and M. L. Fiorotto Overexpression of IGF-I in skeletal muscle of transgenic mice does not prevent unloading-induced atrophy Am J Physiol Endocrinol Metab, September 1, 1998; 275(3): E373 - E379. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. E. Mozdziak, M. L. Greaser, and E. Schultz Myogenin, MyoD, and myosin expression after pharmacologically and surgically induced hypertrophy J Appl Physiol, April 1, 1998; 84(4): 1359 - 1364. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. S. Criswell, V. R. M. Hodgson, E. C. Hardeman, and F. W. Booth Nerve-responsive troponin I slow promoter does not respond to unloading J Appl Physiol, March 1, 1998; 84(3): 1083 - 1087. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. J. Swoap In vivo analysis of the myosin heavy chain IIB promoter region Am J Physiol Cell Physiol, March 1, 1998; 274(3): C681 - C687. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Inobe, I. Inobe, G. R. Adams, K. M. Baldwin, and S.'I. Takeda Effects of microgravity on myogenic factor expressions during postnatal development of rat skeletal muscle J Appl Physiol, May 1, 2002; 92(5): 1936 - 1942. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. R. Vyas, J. J. McCarthy, G. L. Tsika, and R. W. Tsika Multiprotein Complex Formation at the beta Myosin Heavy Chain Distal Muscle CAT Element Correlates with Slow Muscle Expression but Not Mechanical Overload Responsiveness J. Biol. Chem., January 5, 2001; 276(2): 1173 - 1184. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. L. Hallauer and K. E. M. Hastings Coregulation of fast contractile protein transgene and glycolytic enzyme expression in mouse skeletal muscle Am J Physiol Cell Physiol, January 1, 2002; 282(1): C113 - C124. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. A. Huey, R. R. Roy, F. Haddad, V. R. Edgerton, and K. M. Baldwin Transcriptional regulation of the type I myosin heavy chain promoter in inactive rat soleus Am J Physiol Cell Physiol, March 1, 2002; 282(3): C528 - C537. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
