| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Division of Nephrology and Hypertension, Georgetown University Medical Center, Washington, District of Columbia 20007
Micropuncture studies of single nephrons have shown that macula densa solute reabsorption via a furosemide-sensitive pathway activates nitric oxide (NO) generation via neuronal NO synthase (nNOS). This pathway is enhanced during salt loading. We investigated the hypothesis that changes in NO generation via nNOS in the macula densa contribute to changes in whole kidney NO generation and action during alterations in salt intake. Groups of rats (n = 6-10) were equilibrated to high-salt (HS) or low-salt (LS) diets and were administered a vehicle (Veh), 7-nitroindazole (7-NI; a relatively selective inhibitor of nNOS), or furosemide (F; an inhibitor of macula densa solute reabsorption) with volume replacement. Compared with LS, excretion of the NO metabolites, NO2 plus NO3 (NOX) was increased during HS (LS: 9.0 ± 0.5 vs. HS: 15.7 ± 0.8 µmol/24 h; P < 0.001), but this difference was prevented by 7-NI (LS: 7.4 ± 1.3 vs. HS: 9.4 ± 1.6 µmol/24 h; NS). During nonselective blockade of NOS with NG-nitro-L-arginine methyl ester (L-NAME), renal vascular resistance (RVR) increased more in HS than LS (HS: +160 ± 17 vs. LS: +83 ± 10%; P < 0.001). This difference in response to nonselective NOS inhibition was prevented by pretreatment with 7-NI (HS: +28 ± 6 vs. LS: +34 ± 8%; NS) or F with volume replacement (HS: +79 ± 11 vs. LS: +62 ± 4%; NS). In conclusion, compared with salt restriction, HS intake increases NO generation and renal action that depend on nNOS and macula densa solute reabsorption.
nitric oxide; neuronal nitric oxide synthase; 7-nitroindazole; furosemide; renal vascular resistance
This article has been cited by other articles:
|
|
 |
|
  F. Palm, M. L. Onozato, Z. Luo, and C. S. Wilcox Dimethylarginine dimethylaminohydrolase (DDAH): expression, regulation, and function in the cardiovascular and renal systems Am J Physiol Heart Circ Physiol, December 1, 2007; 293(6): H3227 - H3245. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Wang, A. F. Chen, and D. H. Wang Enhanced oxidative stress in kidneys of salt-sensitive hypertension: role of sensory nerves Am J Physiol Heart Circ Physiol, December 1, 2006; 291(6): H3136 - H3143. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. L. Mattson and C. J. Meister Renal cortical and medullary blood flow responses to L-NAME and ANG II in wild-type, nNOS null mutant, and eNOS null mutant mice Am J Physiol Regulatory Integrative Comp Physiol, October 1, 2005; 289(4): R991 - R997. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. Khan, S. Riazi, X. Hu, J. Song, J. B. Wade, and C. A. Ecelbarger Regulation of the renal thiazide-sensitive Na-Cl cotransporter, blood pressure, and natriuresis in obese Zucker rats treated with rosiglitazone Am J Physiol Renal Physiol, August 1, 2005; 289(2): F442 - F450. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Wang, R. J. Roman, J. R. Falck, L. de la Cruz, and J. H. Lombard Effects of high-salt diet on CYP450-4A {omega}-hydroxylase expression and active tone in mesenteric resistance arteries Am J Physiol Heart Circ Physiol, April 1, 2005; 288(4): H1557 - H1565. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Murase, Y. Tian, X. Y. Fang, and J. G. Verbalis Synergistic effects of nitric oxide and prostaglandins on renal escape from vasopressin-induced antidiuresis Am J Physiol Regulatory Integrative Comp Physiol, February 1, 2003; 284(2): R354 - R362. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Y. Na, Y. K. Oh, J. S. Han, K. W. Joo, J. S. Lee, J.-H. Earm, M. A. Knepper, and G.-H. Kim Upregulation of Na+ transporter abundances in response to chronic thiazide or loop diuretic treatment in rats Am J Physiol Renal Physiol, January 1, 2003; 284(1): F133 - F143. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. K. Gupta and B. C. Kone USF-1 and USF-2 trans-repress IL-1beta -induced iNOS transcription in mesangial cells Am J Physiol Cell Physiol, October 1, 2002; 283(4): C1065 - C1072. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Kakoki, A.-P. Zou, and D. L. Mattson The influence of nitric oxide synthase 1 on blood flow and interstitial nitric oxide in the kidney Am J Physiol Regulatory Integrative Comp Physiol, July 1, 2001; 281(1): R91 - R97. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Kitiyakara, T. Chabrashvili, P. Jose, W. J. Welch, and C. S. Wilcox Effects of dietary salt intake on plasma arginine Am J Physiol Regulatory Integrative Comp Physiol, April 1, 2001; 280(4): R1069 - R1075. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. J. Welch, A. Tojo, and C. S. Wilcox Roles of NO and oxygen radicals in tubuloglomerular feedback in SHR Am J Physiol Renal Physiol, May 1, 2000; 278(5): F769 - F776. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. J. Welch, A. Tojo, J.-U. Lee, D. G. Kang, C. G. Schnackenberg, and C. S. Wilcox Nitric oxide synthase in the JGA of the SHR: expression and role in tubuloglomerular feedback Am J Physiol Renal Physiol, July 1, 1999; 277(1): F130 - F138. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
