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-subunit in gills of the teleost
Oreochromis
mossambicus
Department of Zoology, National Chung-Hsing University, Taichung 402, and Institute of Zoology, Academia Sinica, Taipei 115, Taiwan, Republic of China
Three
isoform-specific antibodies, 6F against the
1-isoform of the
avian sodium pump, HERED against the rat
2-isoform, and Ax2 against the
rat 3-isoform, were used to
detect the expression of
Na+-K+-ATPase
-subunits in gills of a teleost, the tilapia
(Oreochromis mossambicus). Tilapia gill tissue
showed positive reactions to antibodies specific for
1- and
3-isoforms. The results of
immunoblots were converted to numerical values (relative intensities)
by image analysis for comparisons. Relative amounts of
1-like isoform alone and
consequently the ratio of
1-like to
3-like isoforms were higher in
gills of seawater-adapted tilapia than in those of freshwater-adapted
ones, indicating that the two isoforms respond differently to
environmental salinities. In the subsequent immunocytochemical experiments, gill mitochondria-rich cells were demonstrated to immunoreact with antibodies specific for
1- and
3-isoforms. 1-like and
3-like isoforms of gill
Na+-K+-ATPase
are suggested to be involved in the ion- and osmoregulation mechanisms
in tilapia. Moreover, differential expressions of two isoforms may be
associated with different functions, secretion and uptake of ions and
acid-base regulation, in gills of seawater- and freshwater-adapted
tilapia.
chloride cell; sodium pump; ion regulation
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