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Am J Physiol Regul Integr Comp Physiol 275: R2070-R2080, 1998;
0363-6119/98 $5.00
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Vol. 275, Issue 6, R2070-R2080, December 1998

Quantification of Ca2+ uptake in the sarcoplasmic reticulum of trout ventricular myocytes

Leif Hove-Madsen, Anna Llach, and Lluis Tort

Department of Physiology and Cell Biology, Faculty of Science, Universitat Autonoma de Barcelona, 08193 Cerdanyola, Barcelona, Spain

We measured Ca2+ uptake by the sarcoplasmic reticulum (SR) in trout ventricular myocytes, measuring indo 1 fluorescence in permeabilized cells or ionic currents in single myocytes subjected to voltage clamp. Titration of the SR Ca2+ pumps with thapsigargin gave a pump site density of 454 pmol/mg cell protein. Lowering the temperature from 20°C to 10 or 5°C reduced the SR Ca2+ uptake rate in permeabilized myocytes by 50 and 63%, respectively. Surprisingly, Ca2+ leak from the SR also decreased with decreasing temperatures. Exposure of single myocytes to 10 mM caffeine (Caf) induced a cell contracture and an inward ionic current. Neither contracture nor current decreased significantly after rest periods of 120 and 320 s. The inward current was due to Ca2+ extrusion by the Na+/Ca2+ exchanger (NCX), and the time integral of the exchange current (INCX) was used to calculate the SR Ca2+ content. This gave a steady-state SR Ca2+ content of 22.5 ± 2.8 amol Ca2+/pF or 750 µM. When the SR was loaded by depolarizing the cell to +50 mV, the Ca2+ content increased with increasing length of the depolarization, reaching a maximum of 52.0 ± 5.9 amol Ca2+/pF. When the cell was depolarized to different voltages for 3 s, a subsequent Caf-induced INCX increased with increasing voltage. At +100 mV, the Ca2+ content was 36.6 ± 3.8 amol/pF, giving a maximal SR Ca2+ uptake rate of 12.2 ± 1.2 amol Ca2+ · pF-1 · s-1 or 417 µM/s. We conclude that maximal SR Ca2+ content and Ca2+ uptake rates can be estimated using specific SR Ca2+ loading protocols. Contrary to the general assumption that contraction in lower vertebrates depends largely on transsarcolemmal Ca2+ fluxes, we found that although the L-type Ca2+ current is insufficient to fully activate contraction, the SR is capable of participating in the regulation of the cytosolic Ca2+ during the excitation-contraction coupling in trout ventricular myocytes.

sodium ion/calcium ion exchange; caffeine; calcium pump; calcium current; lower vertebrate heart ; excitation-contraction coupling


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