AJP - Regu AJP: Regulatory, Integrative and Comparative Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Regul Integr Comp Physiol 276: R575-R585, 1999;
0363-6119/99 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Motoyama, K.
Right arrow Articles by Hotchkiss, R. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Motoyama, K.
Right arrow Articles by Hotchkiss, R. S.
Vol. 276, Issue 2, R575-R585, February 1999

Effect of Ca2+ agonists in the perfused liver: determination via laser scanning confocal microscopy

Kentaro Motoyama1, Irene E. Karl2, M. Wayne Flye1, Dale F. Osborne2, and Richard S. Hotchkiss2,3

1 Department of Surgery; 2 Division of Metabolism, Department of Internal Medicine; and 3 Research Unit, Department of Anesthesiology, Washington University School of Medicine, St. Louis, Missouri 63110

Ca2+ is a critical intracellular second messenger, but few studies have examined Ca2+ signaling in whole organs. The amplitude and frequency of Ca2+ oscillations encode important cellular information. Using laser scanning confocal microscopy in the indo 1 acetoxymethyl ester dye-loaded rat liver, we investigated the effect of various Ca2+ agonists that act at distinct mechanistic sites on Ca2+ signaling. Perfusion with suprathreshold doses of arginine vasopressin (AVP) (2-20 nM) caused a single Ca2+ wave that originated in the pericentral vein region and spread centrifugally to the periportal area. Lower doses of AVP (0.2-2 nM) caused multiple Ca2+ waves and Ca2+ oscillations. Perfusion with ATP (1.4-17.5 µM) caused rapid transient elevations in intracellular free Ca2+ concentration ([Ca2+]i) occurring in isolated hepatocytes or groups of hepatocytes throughout the lobule and were of shorter duration than those due to AVP. Also in contrast to AVP, there was no specific anatomic location within the hepatic lobule that was more susceptible to ATP. Thapsigargin and cyclopiazonic acid did not cause a Ca2+ wave but rather produced a uniform and fairly simultaneous increase in [Ca2+]i in all hepatocytes in the lobule. Perfusion with 14 µM ryanodine produced a single transient spike in [Ca2+]i in a small number (<2%) of hepatocytes. Dantrolene, an inhibitor of Ca2+ release, reduced the increased [Ca2+]i occurring after AVP. Insight into the mechanism of action of these Ca2+-active compounds on Ca2+ signaling in the intact liver is provided.

hepatocytes; ryanodine; thapsigargin; dantrolene; vasopressin


This article has been cited by other articles:


Home page
 Physiol. Rev.Home page
J. C. SAEZ, V. M. BERTHOUD, M. C. BRANES, A. D. MARTINEZ, and E. C. BEYER
Plasma Membrane Channels Formed by Connexins: Their Regulation and Functions
Physiol Rev, October 1, 2003; 83(4): 1359 - 1400.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
M. H. Grayson, R. S. Hotchkiss, I. E. Karl, M. J. Holtzman, and D. D. Chaplin
Intravital microscopy comparing T lymphocyte trafficking to the spleen and the mesenteric lymph node
Am J Physiol Heart Circ Physiol, June 1, 2003; 284(6): H2213 - H2226.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. F. Leite, K. Hirata, T. Pusl, A. D. Burgstahler, K. Okazaki, J. M. Ortega, A. M. Goes, M. A. M. Prado, D. C. Spray, and M. H. Nathanson
Molecular Basis for Pacemaker Cells in Epithelia
J. Biol. Chem., May 3, 2002; 277(18): 16313 - 16323.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online