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Am J Physiol Regul Integr Comp Physiol 277: R1063-R1075, 1999;
0363-6119/99 $5.00
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Vol. 277, Issue 4, R1063-R1075, October 1999

Lesions of the C1 catecholaminergic neurons of the ventrolateral medulla in rats using anti-Dbeta H-saporin

Christopher J. Madden1, Satoru Ito1, Linda Rinaman1, Ronald G. Wiley2, and Alan F. Sved1

1 Department of Neuroscience, University of Pittsburgh, Pittsburgh, Pennsylvania 15260; and 2 Departments of Neurology and Pharmacology, Vanderbilt University, and Veterans Affairs Medical Center, Nashville, Tennessee 37212

Phenylethanolamine-N-methyltransferase (PNMT)-containing neurons in the rostral ventrolateral medulla (RVLM) are believed to play a role in cardiovascular regulation. To determine whether injection of anti-dopamine beta -hydroxylase (Dbeta H)-saporin directly into the RVLM in rats could selectively destroy these cells and thereby provide an approach for evaluating their role in cardiovascular regulation, we studied rats 2 wk after unilateral injection of 21 ng anti-Dbeta H-saporin into the RVLM. There was an ~90% reduction in the number of PNMT-positive neurons in the RVLM, although the number of non-C1, spinally projecting barosensitive neurons of this area was not altered. The A5 cell group was the only other population of Dbeta H-containing cells that was significantly depleted. The depressor response evoked by injection of tyramine into the RVLM was abolished by prior injection of toxin. The pressor response evoked by injection of glutamate into the RVLM was attenuated ipsilateral to the toxin injection but was potentiated contralateral to the toxin injection. Thus anti-Dbeta H-saporin can be used to make selective lesions of PNMT-containing cells, allowing for the evaluation of their role in cardiovascular regulation.

blood pressure; phenylethanolamine-N-methyltransferase; dopamine beta -hydroxylase


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