AJP - Regu Ad Instruments
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Regul Integr Comp Physiol 277: R1205-R1209, 1999;
0363-6119/99 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Clancy, J. S.
Right arrow Articles by Reid, M. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Clancy, J. S.
Right arrow Articles by Reid, M. B.
Vol. 277, Issue 4, R1205-R1209, October 1999

Contractile function is unaltered in diaphragm from mice lacking calcium release channel isoform 3

J. S. Clancy1, H. Takeshima2, S. L. Hamilton1, and M. B. Reid1

1 Baylor College of Medicine, Houston, Texas 77030; and 2 University of Tokyo, Tokyo 113, Japan

Skeletal muscle expresses at least two isoforms of the calcium release channel in the sarcoplasmic reticulum (RyR1 and RyR3). Whereas the function of RyR1 is well defined, the physiological significance of RyR3 is unclear. Some authors have suggested that RyR3 participates in excitation-contraction coupling and that RyR3 may specifically confer resistance to fatigue. To test this hypothesis, we measured contractile function of diaphragm strips from adult RyR3-deficient mice (exon 2-targeted mutation) and their heterozygous and wild-type littermates. In unfatigued diaphragm, there were no differences in isometric contractile properties (twitch characteristics, force-frequency relationships, maximal force) among the three groups. Our fatigue protocol (30 Hz, 0.25 duty cycle, 37°C) depressed force to 25% of the initial force; however, lack of RyR3 did not accelerate the decline in force production. The force-frequency relationship was shifted to higher frequencies and was depressed in fatigued diaphragm; lack of RyR3 did not exaggerate these changes. We therefore provide evidence that RyR3 deficiency does not alter contractile function of adult muscle before, during, or after fatigue.

ryanodine-sensitive calcium channel isoform 3; fatigue; excitation-contraction coupling


This article has been cited by other articles:


Home page
 Am. J. Physiol. Cell Physiol.Home page
C. F. Perez, J. R. Lopez, and P. D. Allen
Expression levels of RyR1 and RyR3 control resting free Ca2+ in skeletal muscle
Am J Physiol Cell Physiol, March 1, 2005; 288(3): C640 - C649.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
A. S. Krupnick, J. Zhu, T. Nguyen, D. Kreisel, K. R. Balsara, E. B. Lankford, C. C. Clark, S. Levine, H. H. Stedman, and J. B. Shrager
Inspiratory loading does not accelerate dystrophy in mdx mouse diaphragm: implications for regenerative therapy
J Appl Physiol, February 1, 2003; 94(2): 411 - 419.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
M. B. Reid, J. Lannergren, and H. Westerblad
Respiratory and Limb Muscle Weakness Induced by Tumor Necrosis Factor-{alpha}: Involvement of Muscle Myofilaments
Am. J. Respir. Crit. Care Med., August 15, 2002; 166(4): 479 - 484.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
R. Rossi, R. Bottinelli, V. Sorrentino, and C. Reggiani
Response to caffeine and ryanodine receptor isoforms in mouse skeletal muscles
Am J Physiol Cell Physiol, August 1, 2001; 281(2): C585 - C594.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online