AJP - Regu Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Regul Integr Comp Physiol 277: R1690-R1696, 1999;
0363-6119/99 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Song, X. M.
Right arrow Articles by Zierath, J. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Song, X. M.
Right arrow Articles by Zierath, J. R.
Vol. 277, Issue 6, R1690-R1696, December 1999

Muscle fiber type specificity in insulin signal transduction

Xiao Mei Song, Jeffrey W. Ryder, Yuichi Kawano, Alexander V. Chibalin, Anna Krook, and Juleen R. Zierath

Department of Clinical Physiology, Gustaf V's Research Institute, Karolinska Hospital, S-171 76 Stockholm; and Department of Physiology and Pharmacology, Karolinska Institute, S-171 77 Stockholm, Sweden

We determined the muscle fiber type-specific response of intracellular signaling proteins to insulin. Epitrochlearis (Epi; 15% type I, 20% type IIa, and 65% type IIb), soleus (84, 16, and 0%), and extensor digitorum longus (EDL; 3, 57, and 40%) muscles from Wistar rats were incubated without or with 120 nM insulin (3-40 min). Peak insulin receptor (IR) tyrosine phosphorylation was reached after 6 (soleus) and 20 (Epi and EDL) min, with sustained activity throughout insulin exposure (40 min). Insulin increased insulin receptor substrate (IRS)-1 and IRS-2 tyrosine phosphorylation and phosphotyrosine-associated phosphatidylinositol (PI)-3-kinase activity to a maximal level after 3-10 min, with subsequent downregulation. Akt kinase phosphorylation peaked at 20 min, with sustained activity throughout insulin exposure. Importantly, the greatest insulin response for all signaling intermediates was observed in soleus, whereas the insulin response between EDL and Epi was similar. Protein expression of the p85alpha -subunit of PI 3-kinase and Akt kinase, but not IR, IRS-1, or IRS-2, was greater in oxidative versus glycolytic muscle. In conclusion, increased function and/or expression of key proteins in the insulin-signaling cascade contribute to fiber type-specific differences in insulin action in skeletal muscle.

insulin receptor; insulin receptor substrate; phosphatidylinositol-3 kinase; Akt kinase


This article has been cited by other articles:


Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
H. Alkhateeb, A. Chabowski, J. F. C. Glatz, J. F. P. Luiken, and A. Bonen
Two phases of palmitate-induced insulin resistance in skeletal muscle: impaired GLUT4 translocation is followed by a reduced GLUT4 intrinsic activity
Am J Physiol Endocrinol Metab, September 1, 2007; 293(3): E783 - E793.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
G. F. Anhe, S. M. Hirabara, T. C. Turrer, L. C. Caperuto, F. F. Anhe, L. M. Ribeiro, A. C. Marcal, C. R. O. Carvalho, R. Curi, U. F. Machado, et al.
Postpartum glycemic homeostasis in early lactating rats is accompanied by transient and specific increase of soleus insulin response through IRS2/AKT pathway
Am J Physiol Regulatory Integrative Comp Physiol, June 1, 2007; 292(6): R2225 - R2233.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
B. B. Dokken, J. A. Sloniger, and E. J. Henriksen
Acute selective glycogen synthase kinase-3 inhibition enhances insulin signaling in prediabetic insulin-resistant rat skeletal muscle
Am J Physiol Endocrinol Metab, June 1, 2005; 288(6): E1188 - E1194.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
H. K.R. Karlsson, K. Hallsten, M. Bjornholm, H. Tsuchida, A. V. Chibalin, K. A. Virtanen, O. J. Heinonen, F. Lonnqvist, P. Nuutila, and J. R. Zierath
Effects of Metformin and Rosiglitazone Treatment on Insulin Signaling and Glucose Uptake in Patients With Newly Diagnosed Type 2 Diabetes: A Randomized Controlled Study
Diabetes, May 1, 2005; 54(5): 1459 - 1467.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
H. Sugita, M. Kaneki, M. Sugita, T. Yasukawa, S. Yasuhara, and J. A. J. Martyn
Burn injury impairs insulin-stimulated Akt/PKB activation in skeletal muscle
Am J Physiol Endocrinol Metab, March 1, 2005; 288(3): E585 - E591.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
Y. Leng, T. L. Steiler, and J. R. Zierath
Effects of Insulin, Contraction, and Phorbol Esters on Mitogen-Activated Protein Kinase Signaling in Skeletal Muscle From Lean and ob/ob Mice
Diabetes, June 1, 2004; 53(6): 1436 - 1444.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
A. D. Krisan, D. E. Collins, A. M. Crain, C. C. Kwong, M. K. Singh, J. R. Bernard, and B. B. Yaspelkis III
Resistance training enhances components of the insulin signaling cascade in normal and high-fat-fed rodent skeletal muscle
J Appl Physiol, May 1, 2004; 96(5): 1691 - 1700.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
E. J. Henriksen, T. R. Kinnick, M. K. Teachey, M. P. O'Keefe, D. Ring, K. W. Johnson, and S. D. Harrison
Modulation of muscle insulin resistance by selective inhibition of GSK-3 in Zucker diabetic fatty rats
Am J Physiol Endocrinol Metab, May 1, 2003; 284(5): E892 - E900.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
T. Miki, K. Minami, L. Zhang, M. Morita, T. Gonoi, T. Shiuchi, Y. Minokoshi, J.-M. Renaud, and S. Seino
ATP-sensitive potassium channels participate in glucose uptake in skeletal muscle and adipose tissue
Am J Physiol Endocrinol Metab, December 1, 2002; 283(6): E1178 - E1184.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
D. G. Hunt and J. L. Ivy
Epinephrine inhibits insulin-stimulated muscle glucose transport
J Appl Physiol, November 1, 2002; 93(5): 1638 - 1643.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
D. G. Hunt, Z. Ding, and J. L. Ivy
Clenbuterol prevents epinephrine from antagonizing insulin-stimulated muscle glucose uptake
J Appl Physiol, March 1, 2002; 92(3): 1285 - 1292.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
S. J. Peters, R. A. Harris, G. J. F. Heigenhauser, and L. L. Spriet
Muscle fiber type comparison of PDH kinase activity and isoform expression in fed and fasted rats
Am J Physiol Regulatory Integrative Comp Physiol, March 1, 2001; 280(3): R661 - R668.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online