AJP - Regu Watch the video to learn how APS reaches out to developing nations.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Regul Integr Comp Physiol 279: R287-R294, 2000;
0363-6119/00 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (22)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Johansson, M.
Right arrow Articles by Powell, T. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Johansson, M.
Right arrow Articles by Powell, T. L.
Vol. 279, Issue 1, R287-R294, July 2000

Na+-K+-ATPase is distributed to microvillous and basal membrane of the syncytiotrophoblast in human placenta

M. Johansson, T. Jansson, and T. L. Powell

Perinatal Center, Department of Physiology and Pharmacology, Göteborg University, S-405 30 Göteborg, Sweden

Despite its importance for placental function, syncytiotrophoblast Na+-K+-ATPase has not been studied in detail. We purified syncytiotrophoblast microvillous (MVM) and basal (BM) membranes from full-term human placenta. Western blotting with isoform-specific antibodies demonstrated the presence of the alpha 1-subunit, but not the alpha 2- or alpha 3-subunits, in MVM and BM. Relative density per unit membrane protein in BM was 48 ± 1% (mean ± SE, n = 4, P < 0.02) of that in the MVM. The activity of Na+-K+-ATPase was lower in BM (1.4 ± 0.14 µmol · mg-1 · min-1, n = 8, P < 0.02) than in MVM (3.9 ± 0.25 µmol · mg-1 · min-1). Immunocytochemistry confirmed the distribution of Na+-K+-ATPase to MVM and BM. These findings suggest that the syncytiotrophoblast represents a type of transporting epithelium different from the classical epithelia found in the small intestine and kidney, where Na+-K+-ATPase is confined to the basolateral membrane only. This unique polarization of the Na+ pump does not, however, preclude a net transcellular transport of Na+ to the fetus.

fetus; sodium transport; 3-O-methylfluorescein phosphate


This article has been cited by other articles:


Home page
 J. Physiol.Home page
S. Roos, N. Jansson, I. Palmberg, K. Saljo, T. L. Powell, and T. Jansson
Mammalian target of rapamycin in the human placenta regulates leucine transport and is down-regulated in restricted fetal growth
J. Physiol., July 1, 2007; 582(1): 449 - 459.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
A. Ericsson, K. Saljo, E. Sjostrand, N. Jansson, P. D. Prasad, T. L. Powell, and T. Jansson
Brief hyperglycaemia in the early pregnant rat increases fetal weight at term by stimulating placental growth and affecting placental nutrient transport
J. Physiol., June 15, 2007; 581(3): 1323 - 1332.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
E. Shibata, R. W. Powers, A. Rajakumar, F. von Versen-Hoynck, M. J. Gallaher, D. L. Lykins, J. M. Roberts, and C. A. Hubel
Angiotensin II decreases system A amino acid transporter activity in human placental villous fragments through AT1 receptor activation
Am J Physiol Endocrinol Metab, November 1, 2006; 291(5): E1009 - E1016.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
A. Ericsson, B. Hamark, T.L. Powell, and T. Jansson
Glucose transporter isoform 4 is expressed in the syncytiotrophoblast of first trimester human placenta
Hum. Reprod., February 1, 2005; 20(2): 521 - 530.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
S. Roos, T. L. Powell, and T. Jansson
Human placental taurine transporter in uncomplicated and IUGR pregnancies: cellular localization, protein expression, and regulation
Am J Physiol Regulatory Integrative Comp Physiol, October 1, 2004; 287(4): R886 - R893.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
K R Page, C J Ashworth, H J McArdle, A M Finch, and M O Nwagwu
Sodium transport across the chorioallantoic membrane of porcine placenta involves the epithelial sodium channel (ENaC)
J. Physiol., March 15, 2003; 547(3): 849 - 857.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
J. P. Granger
Maternal and fetal adaptations during pregnancy: lessons in regulatory and integrative physiology
Am J Physiol Regulatory Integrative Comp Physiol, December 1, 2002; 283(6): R1289 - R1292.
[Full Text] [PDF]

Home page
 DiabetesHome page
T. Jansson, Y. Ekstrand, C. Bjorn, M. Wennergren, and T. L. Powell
Alterations in the Activity of Placental Amino Acid Transporters in Pregnancies Complicated by Diabetes
Diabetes, July 1, 2002; 51(7): 2214 - 2219.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
L. H. Clarson, V. H. J. Roberts, S. L. Greenwood, and A. C. Elliott
ATP-stimulated Ca2+-activated K+ efflux pathway and differentiation of human placental cytotrophoblast cells
Am J Physiol Regulatory Integrative Comp Physiol, April 1, 2002; 282(4): R1077 - R1085.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
S. J. Scheidl, S. Nilsson, M. Kalen, M. Hellstrom, M. Takemoto, J. Hakansson, and P. Lindahl
mRNA Expression Profiling of Laser Microbeam Microdissected Cells from Slender Embryonic Structures
Am. J. Pathol., March 1, 2002; 160(3): 801 - 813.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
K. Leitner, R. Szlauer, I. Ellinger, A. Ellinger, K.-P. Zimmer, and R. Fuchs
Placental Alkaline Phosphatase Expression at the Apical and Basal Plasma Membrane in Term Villous Trophoblasts
J. Histochem. Cytochem., September 1, 2001; 49(9): 1155 - 1164.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online