AJP - Regu Information on EB 2010
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Regul Integr Comp Physiol 279: R1076-R1081, 2000;
0363-6119/00 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (26)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Rajab, P.
Right arrow Articles by Greenhaff, P. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Rajab, P.
Right arrow Articles by Greenhaff, P. L.
Vol. 279, Issue 3, R1076-R1081, September 2000

Skeletal muscle myosin heavy chain isoforms and energy metabolism after clenbuterol treatment in the rat

P. Rajab1, J. Fox1, S. Riaz2, D. Tomlinson2, D. Ball3, and P. L. Greenhaff1

1 School of Biomedical Sciences, University Medical School, Queen's Medical Center, Nottingham NG7 2UH; 2 Dept. of Pharmacology, Queen Mary and Westfield College, University of London, London E1 4NS; and 3 Muscle and Exercise Physiology Group, Manchester Metropolitan University, Alsager ST7 2HL, United Kingdom

Prolonged treatment with the beta 2-adrenoceptor agonist clenbuterol (1-2 mg · kg body mass-1 · day -1) is known to induce the hypertrophy of fast-contracting fibers and the conversion of slow- to fast-contracting fibers. We investigated the effects of administering a lower dose of clenbuterol (250 µg · kg body mass-1 · day -1) on skeletal muscle myosin heavy chain (MyHC) protein isoform content and adenine nucleotide (ATP, ADP, and AMP) concentrations. Male Wistar rats were administered clenbuterol (n = 8) or saline (n = 6) subcutaneously for 8 wk, after which the extensor digitorum longus (EDL) and soleus muscles were removed. We demonstrated an increase of type IIa MyHC protein content in the soleus from ~0.5% in controls to ~18% after clenbuterol treatment (P < 0.05), which was accompanied by an increase in the total adenine nucleotide pool (TAN; ~19%, P < 0.05) and energy charge [E-C = (ATP + 0.5 ADP)/(ATP + ADP + AMP); ~4%; P < 0.05]. In the EDL, a reduction in the content of the less prevalent type I MyHC protein from ~3% in controls to 0% after clenbuterol treatment (P < 0.05) occurred without any alterations in TAN and E-C. These findings demonstrate that the phenotypic changes previously observed in slow muscle after clenbuterol administration at 1-2 mg · kg body mass-1 · day-1 are also observed at a substantially lower dose and are paralleled by concomitant changes in cellular energy metabolism.

beta 2-adrenoceptor agonist; adenosine 5'-triphosphate; energy charge; myosin heavy chain protein composition


This article has been cited by other articles:


Home page
 Physiol. Rev.Home page
G. S. Lynch and J. G. Ryall
Role of {beta}-Adrenoceptor Signaling in Skeletal Muscle: Implications for Muscle Wasting and Disease
Physiol Rev, April 1, 2008; 88(2): 729 - 767.
[Abstract] [Full Text] [PDF]

Home page
 J ANIM SCIHome page
J. M. Gonzalez, J. N. Carter, D. D. Johnson, S. E. Ouellette, and S. E. Johnson
Effect of ractopamine-hydrochloride and trenbolone acetate on longissimus muscle fiber area, diameter, and satellite cell numbers in cull beef cows
J Anim Sci, August 1, 2007; 85(8): 1893 - 1901.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
H. Shi, C. Zeng, A. Ricome, K. M. Hannon, A. L. Grant, and D. E. Gerrard
Extracellular signal-regulated kinase pathway is differentially involved in beta-agonist-induced hypertrophy in slow and fast muscles
Am J Physiol Cell Physiol, May 1, 2007; 292(5): C1681 - C1689.
[Abstract] [Full Text] [PDF]

Home page
 Exp PhysiolHome page
J. G. Burniston, L.-B. Tan, and D. F. Goldspink
Relative myotoxic and haemodynamic effects of the {beta}-agonists fenoterol and clenbuterol measured in conscious unrestrained rats
Exp Physiol, November 1, 2006; 91(6): 1041 - 1049.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
S. W. Jones, D. J. Baker, S. M. Gardiner, T. Bennett, J. A. Timmons, and P. L. Greenhaff
The Effect of the {beta}2-Adrenoceptor Agonist Prodrug BRL-47672 on Cardiovascular Function, Skeletal Muscle Myosin Heavy Chain, and MyoD Expression in the Rat
J. Pharmacol. Exp. Ther., December 1, 2004; 311(3): 1225 - 1231.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
S. Busquets, M. T. Figueras, G. Fuster, V. Almendro, R. Moore-Carrasco, E. Ametller, J. M. Argiles, and F. J. Lopez-Soriano
Anticachectic Effects of Formoterol: A Drug for Potential Treatment of Muscle Wasting
Cancer Res., September 15, 2004; 64(18): 6725 - 6731.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
J. G. Ryall, P. Gregorevic, D. R. Plant, M. N. Sillence, and G. S. Lynch
beta 2-Agonist fenoterol has greater effects on contractile function of rat skeletal muscles than clenbuterol
Am J Physiol Regulatory Integrative Comp Physiol, December 1, 2002; 283(6): R1386 - R1394.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
B. L. Awede, J.-P. Thissen, and J. Lebacq
Role of IGF-I and IGFBPs in the changes of mass and phenotype induced in rat soleus muscle by clenbuterol
Am J Physiol Endocrinol Metab, January 1, 2002; 282(1): E31 - E37.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online