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Am J Physiol Regul Integr Comp Physiol 279: R1996-R2003, 2000;
0363-6119/00 $5.00
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Vol. 279, Issue 6, R1996-R2003, December 2000

Na+/H+ exchange in mosquito Malpighian tubules

D. H. Petzel

Department of Biomedical Sciences, Creighton University School of Medicine, Omaha, Nebraska 68178

Fluid secretion and intracellular pH were measured in isolated mosquito Malpighian tubules to determine the presence of Na+/H+ exchange. Rates of fluid secretion by individual Malpighian tubules in vitro were inhibited by 78% of control in the presence of 100 µM 5-(N-ethyl-n-isopropyl)-amiloride (EIPA), a specific inhibitor of Na+/H+ exchange. Steady-state intracellular pH was measured microfluorometrically by using 2',7'-bis(2-carboxyethyl)-5(6)-carboxyfluorescein in individual Malpighian tubules. Bathing the Malpighian tubules in 0 mM extracellular Na+ or in the presence of 100 µM EIPA reduced the steady-state intracellular pH by 0.5 pH units. Stimulation of the Na+/H+ exchanger by using the NH4Cl pulse technique resulted in a rate of recovery from the NH4Cl-induced acute acid load of 8.7 ± 1.0 × 10-3 pH/s. The rates of recovery of intracellular pH after the acute acid load in the absence of extracellular Na+ or in the presence of 100 µM EIPA were 0.7 ± 0.6 and -0.3 ± 0.3 × 10-3 pH/s, respectively. These results indicate that mosquito Malpighian tubules possess a Na+/H+ exchanger.

Aedes aegypti; microfluorescence; intracellular pH; 2',7'-bis(2-carboxyethyl)-5(6)-carboxyfluorescein; amiloride


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