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Am J Physiol Regul Integr Comp Physiol 280: R527-R535, 2001;
0363-6119/01 $5.00
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Vol. 280, Issue 2, R527-R535, February 2001

Effects of 17beta -estradiol on levels and distribution of metallothionein and zinc in squirrelfish

E. David Thompson1, Per-Erik Olsson2, Gregory D. Mayer1, Carl Haux3, Patrick J. Walsh4, Erin Burge1, and Christer Hogstrand1,4

1 T.H. Morgan School of Biological Sciences, University of Kentucky, Lexington, Kentucky 40506-0225; 2 Division of Physiology, Department of Cellular and Molecular Biology, Umeå University, SE-901 87 Umeå, Sweden; 3 European Commission, Joint Research Centre, Environment Institute, TP 460, I-21020 Ispra, Italy; and 4 Division of Marine Biology and Fisheries and National Institute of Environmental Health Sciences Marine and Freshwater Biomedical Science Center, Rosenstiel School of Marine and Atmospheric Sciences, University of Miami, Miami, Florida 33149

Females of the squirrelfish family (Holocentridae) accumulate higher levels of zinc in the liver than any other known animal. This zinc accumulation is made possible by high expression of the zinc-binding protein, metallothionein (MT). In the present study, the squirrelfish (Holocentrus ascensionis) MT cDNA was cloned and sequenced. The deduced amino acid sequence was very similar to other teleost MT. The role of estrogens on zinc metabolism was investigated by injecting male and immature female squirrelfish with 17beta -estradiol (E2). E2 treatment triggered transient increases in plasma zinc and vitellogenin (VTG) levels, and both of these variables showed very similar time courses. These results suggest that E2 is responsible for the large hepatoovarian translocation of zinc observed in female squirrelfish and that VTG might be a vehicle for zinc. E2 did not directly alter the levels of zinc or MT mRNA in the liver. However, the hepatic MT protein concentration increased differentially in the nuclear fraction. Thus E2 is probably responsible for the association of MT with the nuclear fraction previously observed in untreated mature female squirrelfish.

zinc; metallothionein complimentary deoxyribonucleic acid; reproduction; endocrinology; teleost




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