AJP - Regu Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Regul Integr Comp Physiol 280: R1095-R1104, 2001;
0363-6119/01 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (12)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Moellenhoff, E.
Right arrow Articles by Unger, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Moellenhoff, E.
Right arrow Articles by Unger, T.
Vol. 280, Issue 4, R1095-R1104, April 2001

Effect of repetitive icv injections of ANG II on c-Fos and AT1-receptor expression in the rat brain

Eva Moellenhoff1,2, Annegret Blume1,2, Juraj Culman1,2, Bimal Chatterjee3, Thomas Herdegen1, Christine J. Lebrun1,2, and Thomas Unger1,2

1 Institute of Pharmacology, University of Kiel, 24105 Kiel; 2 German Institute for High Blood Pressure Research, 69120 Heidelberg; and 3 Gesselschaft Für Strahlenforschling-National Research Center for Environment and Health, Institute of Mammalian Genetics, 85764 Neuherberg, Germany

ANG II has been implicated in neuroplastic processes via stimulation of inducible transcription factors (ITF) in the brain. In the present study, we investigated the effects of acute vs. repetitive once daily intracerebroventricular injections of ANG II for 7 days on the expression of ITF and constitutive transcription factor (CTF) and the AT1 receptor in the median preoptic area (MnPO), the subfornical organ (SFO), and the hypothalamic paraventricular (PVN) and supraoptic nuclei (SON). After repetitive injections, the expression of c-Fos declined by ~50% in MnPO, SFO, PVN, and SON compared with controls injected once. The desensitization of c-Fos occurred on the transcriptional level as shown in the SON by RT-PCR. Apart from a novel expression of c-Jun in the SON, the ITF c-Jun, JunB, JunD, and Krox-24 did not change after repetitive stimulation. Neither were the CTF, calcium response element binding protein, activating transcription factor 2, and serum response factor altered after repetitive vs. single injections of ANG II. The AT1 receptor was coexpressed with c-Fos/c-Jun. Immunohistochemical stainings suggest an increase in AT1-receptor number in MnPO, SFO, PVN, and SON on chronic stimulation compared with once-injected controls. These findings demonstrate that repetitive periventricular stimulation with ANG II essentially alters the expression of transcription factors compared with acute stimulation and suggest c-Fos and c-Jun as major intermediates of the AT1-receptor transcription.

chronic stimulation; desensitization; inducible transcription factor; constitutive transcription factor; angiotensin AT1 receptor; intracerebroventricular


This article has been cited by other articles:


Home page
 Circ. Res.Home page
D. Liu, L. Gao, S. K. Roy, K. G. Cornish, and I. H. Zucker
Neuronal Angiotensin II Type 1 Receptor Upregulation in Heart Failure: Activation of Activator Protein 1 and Jun N-Terminal Kinase
Circ. Res., October 27, 2006; 99(9): 1004 - 1011.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
L. Gao, W. Wang, Y.-L. Li, H. D. Schultz, D. Liu, K. G. Cornish, and I. H. Zucker
Sympathoexcitation by central ANG II: Roles for AT1 receptor upregulation and NAD(P)H oxidase in RVLM
Am J Physiol Heart Circ Physiol, May 1, 2005; 288(5): H2271 - H2279.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
J. Tan, H. Wang, and F. H. H. Leenen
Increases in brain and cardiac AT1 receptor and ACE densities after myocardial infarct in rats
Am J Physiol Heart Circ Physiol, May 1, 2004; 286(5): H1665 - H1671.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
O. Skott
Body sodium and volume homeostasis
Am J Physiol Regulatory Integrative Comp Physiol, July 1, 2003; 285(1): R14 - R18.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online