AJP - Regu Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Regul Integr Comp Physiol 280: R959-R967, 2001;
0363-6119/01 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (7)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Andrew, P. S.
Right arrow Articles by Kaufman, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Andrew, P. S.
Right arrow Articles by Kaufman, S.
Vol. 280, Issue 4, R959-R967, April 2001

Nitric oxide increases fluid extravasation from the splenic circulation of the rat

Peter S. Andrew, Yiming Deng, Richard Sultanian, and Susan Kaufman

Departments of Physiology and Medicine, University of Alberta, Edmonton, Alberta T6G 2S2, Canada

We hypothesized that nitric oxide (NO) contributes to intrasplenic fluid extravasation by inducing greater relaxation in splenic resistance arteries than veins such that intrasplenic microvascular pressure (PC) rises. Fluid efflux was estimated by measuring the difference between splenic blood inflow and outflow. Intrasplenic infusion of the NO donor S-nitroso-N-acetylpenicillamine (SNAP) (0.3 µg · 10 µl-1 · min-1) caused a significant increase in intrasplenic fluid efflux (baseline: 0.8 ± 0.4 ml/min, n = 10 vs. peak rise during SNAP infusion: 1.3 ± 0.4 ml/min, n = 10; P < 0.05). Intrasplenic PC was measured in the isolated, blood-perfused rat spleen. Intrasplenic infusion of SNAP (0.1 µg · 10 µl-1 · min-1) caused a significant increase in PC (saline: 10.9 ± 0.2 mmHg, n = 3 vs. SNAP: 12.2 ± 0.2 mmHg, n = 3; P < 0.05). Vasoreactivity of preconstricted splenic resistance vessels to sodium nitroprusside (SNP) (1 × 10-12-1 × 10-4 M) and SNAP (1 × 10-10-3 × 10-4 M) was investigated with the use of a wire myograph system. Significantly greater relaxation of arterioles than of venules occurred with both SNP (%maximal vasorelaxation: artery 96 ± 2.3, n = 9 vs. vein 26 ± 1.9, n = 10) and SNAP (%maximal vasorelaxation: artery 50 ± 3.5, n = 11 vs. vein 32 ± 1.7, n = 8). These results are consistent with our proposal that differential vasoreactivity of splenic resistance arteries and veins to NO elevates intrasplenic PC and increases fluid extravasation into the systemic lymphatic system.

spleen; microvascular pressure; blood flow


This article has been cited by other articles:


Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
H. Ehmke
Inducing vasoconstriction by activating guanylyl cyclase
Am J Physiol Regulatory Integrative Comp Physiol, June 1, 2003; 284(6): R1376 - R1377.
[Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
S. Kaufman and J. Levasseur
Effect of portal hypertension on splenic blood flow, intrasplenic extravasation and systemic blood pressure
Am J Physiol Regulatory Integrative Comp Physiol, June 1, 2003; 284(6): R1580 - R1585.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
P. S. Andrew and S. Kaufman
Guanylyl cyclase mediates ANP-induced vasoconstriction of murine splenic vessels
Am J Physiol Regulatory Integrative Comp Physiol, June 1, 2003; 284(6): R1567 - R1571.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
Z. L. S. Brookes and S. Kaufman
Myogenic responses and compliance of mesenteric and splenic vasculature in the rat
Am J Physiol Regulatory Integrative Comp Physiol, June 1, 2003; 284(6): R1604 - R1610.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online