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Am J Physiol Regul Integr Comp Physiol 280: R1269-R1275, 2001;
0363-6119/01 $5.00
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Vol. 280, Issue 5, R1269-R1275, May 2001

Expression of endothelial nitric oxide synthase in the postnatal developing porcine kidney

Michael J. Solhaug1, Usa Kullaprawithaya1,2, Xui Q. Dong1, and Ke-Wen Dong3

1 Departments of Pediatrics, Physiological Sciences, and 3 Obstetrics and Gynecology, Eastern Virginia Medical School, Norfolk 23501, and 2 Department of Chemistry, Old Dominion University, Norfolk, Virginia 23529

The postnatal pattern of renal endothelial nitric oxide synthase (eNOS) is unknown. The purpose of this study was to characterize eNOS expression during maturation and compare this to neuronal NOS (nNOS). The experiments measured whole kidney eNOS mRNA expression by RT-PCR and protein content by Western blot, as well as cortical and medullary protein content in piglets at selected postnatal ages and in adult pigs. Whole kidney eNOS mRNA was compared with nNOS. Whole kidney eNOS expression decreased from the newborn to its lowest at 7 days, returning by 14 days to adult levels. This eNOS mRNA pattern contrasted with nNOS, which was highest at birth, and progressively decreased to its lowest level in the adult. At birth, cortical eNOS protein was greater than medullary, contrasting with the adult pattern of equivalent levels. In conclusion eNOS is developmentally regulated during early renal maturation and may critically participate in renal function during this period. The eNOS developmental pattern differs from nNOS, suggesting that these isoforms may have different regulatory factors and functional contributions in the postnatal kidney.

neuronal; gene expression; protein content; development


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