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Am J Physiol Regul Integr Comp Physiol 281: R10-R18, 2001;
0363-6119/01 $5.00
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Vol. 281, Issue 1, R10-R18, July 2001

Ouabain-insensitive acidification by dopamine in renal OK cells: primary control of the Na+/H+ exchanger

Pedro Gomes, M. A. Vieira-Coelho, and P. Soares-da-Silva

Faculty of Medicine, Institute of Pharmacology and Therapeutics, 4200 Porto, Portugal

The present study was aimed at evaluating the role of D1- and D2-like receptors and investigating whether inhibition of Na+ transepithelial flux by dopamine is primarily dependent on inhibition of the apical Na+/H+ exchanger, inhibition of the basolateral Na+-K+-ATPase, or both. The data presented here show that opossum kidney cells are endowed with D1- and D2-like receptors, the activation of the former, but not the latter, accompanied by stimulation of adenylyl cyclase (EC50 = 220 ± 2 nM), marked intracellular acidification (IC50 = 58 ± 2 nM), and attenuation of amphotericin B-induced decreases in short-circuit current (28.6 ± 4.5% reduction) without affecting intracellular pH recovery after CO2 removal. These results agree with the view that dopamine, through the activation of D1- but not D2-like receptors, inhibits both the Na+/H+ exchanger (0.001933 ± 0.000121 vs. 0.000887 ± 0.000073 pH unit/s) and Na+-K+-ATPase without interfering with the Na+-independent HCO<UP><SUB>3</SUB><SUP>−</SUP></UP> transporter. It is concluded that dopamine, through the action of D1-like receptors, inhibits both the Na+/H+ exchanger and Na+-K+-ATPase, but its marked acidifying effects result from inhibition of the Na+/H+ exchanger only, without interfering with the Na+-independent HCO<UP><SUB>3</SUB><SUP>−</SUP></UP> transporter and Na+-K+-ATPase.

opossum kidney, intracellular pH; Na+-K+-ATPase


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