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Neurosciences, Ottawa Health Research Institute, Ottawa Hospital Civic Site, and University of Ottawa, Ottawa, Ontario, K1Y 4E9, Canada
We used patch-clamp
recordings in slice preparations from Sprague-Dawley rats to evaluate
responses of 20 spinal-projecting neurons in the dorsal paraventricular
nucleus (PVN) to electrical stimulation in suprachiasmatic nucleus
(SCN). Neurons containing a retrograde label transported from the
thoracic (T1-T4) intermediolateral column
displayed three intrinsic properties that collectively allowed
distinction from neighboring parvocellular or magnocellular cells: a
low-input resistance, a hyperpolarization-activated time-dependent inward rectification, and a low-threshold calcium conductance. Twelve
of fifteen cells tested responded to electrical stimulation in SCN. All
of 10 cells tested in media containing
2,3,-dioxo-6-nitro-1,2,3,4-tetrahydrobenzo[f]quinoxaline-7-sulfonamide disodium (5 µM) and
D(
)-2-amino-5-phosphonopentanoic acid (20 µM)
responded with constant latency (11.4 ± 0.7 ms) inhibitory postsynaptic potentials, able to follow 20- to 50-Hz stimulation and
blockable with bicuculline (20 µM). By contrast, all eight cells
tested in the presence of bicuculline demonstrated constant latency
(9.8 ± 0.6 ms) excitatory postsynaptic potentials that followed
at 20-50 Hz and featured both
non-N-methyl-D-aspartate (NMDA) and NMDA
receptor-mediated components. We conclude that both GABAergic and
glutamatergic neurons in SCN project directly to spinal-projecting
neurons in the dorsal PVN.
retrograde labeling; rhodamine microspheres; intrinsic conductances; hyperpolarization-activated inward rectification; T current
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