AJP - Regu Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Regul Integr Comp Physiol 281: R1394-R1400, 2001;
0363-6119/01 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (11)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Emch, G. S.
Right arrow Articles by Rogers, R. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Emch, G. S.
Right arrow Articles by Rogers, R. C.
Vol. 281, Issue 5, R1394-R1400, November 2001

TNF-alpha -induced c-Fos generation in the nucleus of the solitary tract is blocked by NBQX and MK-801

Gregory S. Emch, Gerlinda E. Hermann, and Richard C. Rogers

Department of Neuroscience, The Ohio State University, Columbus, Ohio 43210

Previous studies have shown that identified neurons of the nucleus of the solitary tract (NST) are excited by the cytokine tumor necrosis factor-alpha (TNF-alpha ). Vagal afferent connections with the NST are predominantly glutaminergic. Therefore, we hypothesized that TNF-alpha effects on NST neurons may be via modulation of glutamate neurotransmission. The present study used activation of the immediate early gene product c-Fos as a marker for neuronal activation in the NST. c-Fos expression was evaluated after microinjections of TNF-alpha in the presence or absence of either the alpha -amino-3-hydroxy-5-methylisoxazole-4-propionic acid receptor antagonist 1,2,3,4-tetrahydro-6-nitro-2,3-dioxo-benzo[f]quinoxaline-7-sulfonamide disodium (NBQX) or the N-methyl-D- aspartate (NMDA) antagonist MK-801. To assess the specificity of the interaction between TNF-alpha and glutamate, c-Fos expression was also evaluated after injection of oxytocin (OT) (which has a direct excitatory effect in this area of the brain stem) in the presence and absence of NBQX or MK-801. c-Fos labeling was significantly increased in the NST after TNF-alpha exposure. Coinjection of either NBQX or MK-801 with TNF-alpha prevented significant c-Fos induction in the NST. Microinjections of OT also induced significant NST c-Fos elevation, but this expression was unaffected by coinjection of either antagonist with OT. These data lead us to conclude that TNF-alpha activation of NST neurons depends on glutamate and such an interaction is not generalized to all agonists that act on the NST.

cytokines; brain stem; dorsal vagal complex; tumor necrosis factor-alpha


This article has been cited by other articles:


Home page
 Exp PhysiolHome page
M. Pickering, D. Cumiskey, and J. J O'Connor
Actions of TNF-{alpha} on glutamatergic synaptic transmission in the central nervous system
Exp Physiol, September 1, 2005; 90(5): 663 - 670.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
G. E. Hermann, J. S. Nasse, and R. C. Rogers
{alpha}-1 adrenergic input to solitary nucleus neurones: calcium oscillations, excitation and gastric reflex control
J. Physiol., January 15, 2005; 562(2): 553 - 568.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
R. C. Rogers, R. A. Travagli, and G. E. Hermann
Noradrenergic neurons in the rat solitary nucleus participate in the esophageal-gastric relaxation reflex
Am J Physiol Regulatory Integrative Comp Physiol, August 1, 2003; 285(2): R479 - R489.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
G. E. Hermann, C. A. Tovar, and R. C. Rogers
LPS-induced suppression of gastric motility relieved by TNFR:Fc construct in dorsal vagal complex
Am J Physiol Gastrointest Liver Physiol, September 1, 2002; 283(3): G634 - G639.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online