|
|
||||||||
Department of Biology Sciences, University of Alberta, Edmonton, Alberta, Canada T6G 2E9
Fluorescently labeled peanut lectin agglutinin (PNA-FITC) was used to identify a subtype of mitochondria-rich (MR) cells in the gills of freshwater rainbow trout. In situ binding of PNA-FITC was visualized by inverted fluorescence microscopy and found to bind to cells on the trailing edge of the filament epithelium as demonstrated by differential interference contrast optics. The amount of PNA-FITC binding on the filament epithelium increased with cortisol pretreatment concomitant with an increased chloride cell fractional area as demonstrated by scanning electron microscopy. Dispersed gill cells were isolated by trypsinization and separated using a discontinuous Percoll density gradient. Cells migrating to the 1.06-1.09 g/ml interface were found to be MR as demonstrated by staining with the vital mitochondrial dye 4-(4-(dimethylamino)styryl)-N-methylpyridinium iodide and transmission electron microscopy (TEM). However, only ~40% of the MR cells were found to bind PNA-FITC. Cortisol pretreatment increased the relative numbers of MR cells isolated from the dispersed gill cell population, but the relative proportions of PNA binding cells remained unchanged. Ultrastructural analysis of isolated cells in the TEM demonstrated that the MR cell fraction was comprised of a mixed population of chloride cells and pavement cells.
chloride cells; pavement cells; peanut lectin; peanut lectin agglutinin; scanning electron microscopy; transmission electron microscopy; fish
This article has been cited by other articles:
![]() |
D. Mitrovic, A. Dymowska, G. E. Nilsson, and S. F. Perry Physiological consequences of gill remodeling in goldfish (Carassius auratus) during exposure to long-term hypoxia Am J Physiol Regulatory Integrative Comp Physiol, July 1, 2009; 297(1): R224 - R234. [Abstract] [Full Text] [PDF] |
||||
![]() |
M. Inokuchi, J. Hiroi, S. Watanabe, P.-P. Hwang, and T. Kaneko Morphological and functional classification of ion-absorbing mitochondria-rich cells in the gills of Mozambique tilapia J. Exp. Biol., April 1, 2009; 212(7): 1003 - 1010. [Abstract] [Full Text] [PDF] |
||||
![]() |
J.-L. Horng, L.-Y. Lin, and P.-P. Hwang Functional regulation of H+-ATPase-rich cells in zebrafish embryos acclimated to an acidic environment Am J Physiol Cell Physiol, April 1, 2009; 296(4): C682 - C692. [Abstract] [Full Text] [PDF] |
||||
![]() |
W.-J. Chang, J.-L. Horng, J.-J. Yan, C.-D. Hsiao, and P.-P. Hwang The transcription factor, glial cell missing 2, is involved in differentiation and functional regulation of H+-ATPase-rich cells in zebrafish (Danio rerio) Am J Physiol Regulatory Integrative Comp Physiol, April 1, 2009; 296(4): R1192 - R1201. [Abstract] [Full Text] [PDF] |
||||
![]() |
S. K. Parks, M. Tresguerres, and G. G. Goss Cellular mechanisms of Cl- transport in trout gill mitochondrion-rich cells Am J Physiol Regulatory Integrative Comp Physiol, April 1, 2009; 296(4): R1161 - R1169. [Abstract] [Full Text] [PDF] |
||||
![]() |
S. S. Madsen, P. Kiilerich, and C. K. Tipsmark Multiplicity of expression of Na+,K+-ATPase {alpha}-subunit isoforms in the gill of Atlantic salmon (Salmo salar): cellular localisation and absolute quantification in response to salinity change J. Exp. Biol., January 1, 2009; 212(1): 78 - 88. [Abstract] [Full Text] [PDF] |
||||
![]() |
D. H. Evans Teleost fish osmoregulation: what have we learned since August Krogh, Homer Smith, and Ancel Keys Am J Physiol Regulatory Integrative Comp Physiol, August 1, 2008; 295(2): R704 - R713. [Abstract] [Full Text] [PDF] |
||||
![]() |
T. Nakada, K. Hoshijima, M. Esaki, S. Nagayoshi, K. Kawakami, and S. Hirose Localization of ammonia transporter Rhcg1 in mitochondrion-rich cells of yolk sac, gill, and kidney of zebrafish and its ionic strength-dependent expression Am J Physiol Regulatory Integrative Comp Physiol, October 1, 2007; 293(4): R1743 - R1753. [Abstract] [Full Text] [PDF] |
||||
![]() |
S. K. Parks, M. Tresguerres, and G. G. Goss Interactions between Na+ channels and Na+-HCO3- cotransporters in the freshwater fish gill MR cell: a model for transepithelial Na+ uptake Am J Physiol Cell Physiol, February 1, 2007; 292(2): C935 - C944. [Abstract] [Full Text] [PDF] |
||||
![]() |
F. Galvez, D. Wong, and C. M. Wood Cadmium and calcium uptake in isolated mitochondria-rich cell populations from the gills of the freshwater rainbow trout Am J Physiol Regulatory Integrative Comp Physiol, July 1, 2006; 291(1): R170 - R176. [Abstract] [Full Text] [PDF] |
||||
![]() |
L.-Y. Lin, J.-L. Horng, J. G. Kunkel, and P.-P. Hwang Proton pump-rich cell secretes acid in skin of zebrafish larvae Am J Physiol Cell Physiol, February 1, 2006; 290(2): C371 - C378. [Abstract] [Full Text] [PDF] |
||||
![]() |
D. H. Evans, P. M. Piermarini, and K. P. Choe The Multifunctional Fish Gill: Dominant Site of Gas Exchange, Osmoregulation, Acid-Base Regulation, and Excretion of Nitrogenous Waste Physiol Rev, January 1, 2005; 85(1): 97 - 177. [Abstract] [Full Text] [PDF] |
||||
![]() |
L.-Y. Lin and P.-P. Hwang Mitochondria-rich cell activity in the yolk-sac membrane of tilapia (Oreochromis mossambicus) larvae acclimatized to different ambient chloride levels J. Exp. Biol., March 15, 2004; 207(8): 1335 - 1344. [Abstract] [Full Text] [PDF] |
||||
![]() |
G. S. Hawkings, F. Galvez, and G. G. Goss Seawater acclimation causes independent alterations in Na+/K+- and H+-ATPase activity in isolated mitochondria-rich cell subtypes of the rainbow trout gill J. Exp. Biol., February 22, 2004; 207(6): 905 - 912. [Abstract] [Full Text] [PDF] |
||||
![]() |
J. B. Pritchard The gill and homeostasis: transport under stress Am J Physiol Regulatory Integrative Comp Physiol, December 1, 2003; 285(6): R1269 - R1271. [Full Text] [PDF] |
||||
![]() |
S. D. Reid, G. S. Hawkings, F. Galvez, and G. G. Goss Localization and characterization of phenamil-sensitive Na+ influx in isolated rainbow trout gill epithelial cells J. Exp. Biol., February 1, 2003; 206(3): 551 - 559. [Abstract] [Full Text] [PDF] |
||||
![]() |
F. Galvez, S. D. Reid, G. Hawkings, and G. G. Goss Isolation and characterization of mitochondria-rich cell types from the gill of freshwater rainbow trout Am J Physiol Regulatory Integrative Comp Physiol, March 1, 2002; 282(3): R658 - R668. [Abstract] [Full Text] [PDF] |
||||
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |