AJP - Regu Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Regul Integr Comp Physiol 281: R1808-R1816, 2001;
0363-6119/01 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (17)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Chen, Y.-F.
Right arrow Articles by Zou, A.-P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chen, Y.-F.
Right arrow Articles by Zou, A.-P.
Vol. 281, Issue 6, R1808-R1816, December 2001

Oxidative stress enhances the production and actions of adenosine in the kidney

Ya-Fei Chen, Pin-Lan Li, and Ai-Ping Zou

Departments of Physiology and Pharmacology and Toxicology, Medical College of Wisconsin, Milwaukee, Wisconsin 53226

The purpose of this study was to determine whether superoxide anions (O<UP><SUB>2</SUB><SUP>−</SUP></UP>·) activate 5'-nucleotidase (5'-ND), thereby increasing the production of renal adenosine and regulating renal function. Using HPLC analysis, we found that incubation of renal tissue homogenate with the O<UP><SUB>2</SUB><SUP>−</SUP></UP>· donor KO2 doubled adenosine production and increased the maximal reaction velocity of 5'-ND from 141 to 192 nmol · min-1 · mg protein-1. The O<UP><SUB>2</SUB><SUP>−</SUP></UP>·-generating system, xanthine/xanthine oxidase increased the maximal reaction velocity of 5'-ND from 122 to 204 nmol · min-1 · mg protein-1. Superoxide dismutase (SOD) with catalase produced a concentration-dependent reduction of 5'-ND activity in renal tissue homogenate, while the SOD inhibitor diethyldithiocarbamic acid significantly increased 5'-ND activity. Inhibition of disulfide bond formation by thioredoxin or thioredoxin reductase significantly decreased xanthine/xanthine oxidase-induced activation of renal 5'-ND. In in vivo experiments, inhibition of SOD by diethyldithiocarbamic acid (0.5 mg · kg-1 · min-1 iv) enhanced renal vasoconstriction induced by endogenously produced adenosine and increased renal tissue adenosine concentrations under control condition and in ischemia and reperfusion. We conclude that oxidative stress activates 5'-ND and increases adenosine production in the kidney and that this redox regulatory mechanism of adenosine production is important in the control of renal vascular tone and glomerular perfusion.

redox signaling; renal hemodynamics; reactive oxygen species; nucleotide


This article has been cited by other articles:


Home page
 Am. J. Physiol. Renal Physiol.Home page
M. Franco, R. Bautista, O. Perez-Mendez, L. Gonzalez, U. Pacheco, L. G. Sanchez-Lozada, J. Santamaria, E. Tapia, R. Monreal, and F. Martinez
Renal interstitial adenosine is increased in angiotensin II-induced hypertensive rats
Am J Physiol Renal Physiol, January 1, 2008; 294(1): F84 - F92.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
L. T. de Richelieu, C. M. Sorensen, N.-H. Holstein-Rathlou, and M. Salomonsson
NO-independent mechanism mediates tempol-induced renal vasodilation in SHR
Am J Physiol Renal Physiol, December 1, 2005; 289(6): F1227 - F1234.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
W. J. Welch, J. Blau, H. Xie, T. Chabrashvili, and C. S. Wilcox
Angiotensin-induced defects in renal oxygenation: role of oxidative stress
Am J Physiol Heart Circ Physiol, January 1, 2005; 288(1): H22 - H28.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Clin. Nutr.Home page
S J. James, P. Cutler, S. Melnyk, S. Jernigan, L. Janak, D. W Gaylor, and J. A Neubrander
Metabolic biomarkers of increased oxidative stress and impaired methylation capacity in children with autism
Am. J. Clinical Nutrition, December 1, 2004; 80(6): 1611 - 1617.
[Abstract] [Full Text] [PDF]

Home page
 HypertensionHome page
M. W. Brands, T. D. Bell, and B. Gibson
Nitric Oxide May Prevent Hypertension Early in Diabetes by Counteracting Renal Actions of Superoxide
Hypertension, January 1, 2004; 43(1): 57 - 63.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
Y.-F. Chen, A. W. Cowley Jr., and A.-P. Zou
Increased H2O2 counteracts the vasodilator and natriuretic effects of superoxide dismutation by tempol in renal medulla
Am J Physiol Regulatory Integrative Comp Physiol, October 1, 2003; 285(4): R827 - R833.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
Z.-Z. Yang, A. Y. Zhang, F.-X. Yi, P.-L. Li, and A.-P. Zou
Redox regulation of HIF-1alpha levels and HO-1 expression in renal medullary interstitial cells
Am J Physiol Renal Physiol, June 1, 2003; 284(6): F1207 - F1215.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
N. Li, F.-X. Yi, J. L. Spurrier, C. A. Bobrowitz, and A.-P. Zou
Production of superoxide through NADH oxidase in thick ascending limb of Henle's loop in rat kidney
Am J Physiol Renal Physiol, June 1, 2002; 282(6): F1111 - F1119.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online