AJP - Regu AJP: Lung Cellular and Molecular Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Regul Integr Comp Physiol 282: R439-R444, 2002; doi:10.1152/ajpregu.00512.2001
0363-6119/02 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (23)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Penner, G.
Right arrow Articles by Hasselgren, P.-O.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Penner, G.
Right arrow Articles by Hasselgren, P.-O.
Vol. 282, Issue 2, R439-R444, February 2002

C/EBP DNA-binding activity is upregulated by a glucocorticoid-dependent mechanism in septic muscle

Gail Penner1, Gyu Gang1, Xiaoyan Sun1,2, Curtis Wray1, and Per-Olof Hasselgren1

1 Department of Surgery, University of Cincinnati, Cincinnati 45267-0558; and 2 Shriners Hospitals for Children, Cincinnati, Ohio 45229-3095

Sepsis-induced muscle cachexia is associated with increased expression of several genes in the ubiquitin-proteasome proteolytic pathway, but little is known about the activation of transcription factors in skeletal muscle during sepsis. We tested the hypothesis that sepsis upregulates the expression and activity of the transcription factors CCAAT/enhancer binding protein (C/EBP)-beta and -delta in skeletal muscle. Sepsis was induced in rats by cecal ligation and puncture, and control rats were sham operated. C/EBP-beta and -delta DNA-binding activity was determined by electrophoretic mobility shift assay and supershift analysis. In addition, C/EBP-beta and -delta nuclear protein levels were determined by Western blot analysis. Sepsis resulted in increased DNA-binding activity of C/EBP, and supershift analysis suggested that this reflected activation of the beta - and delta -isoforms of C/EBP. Concomitantly, C/EBP-beta and -delta protein levels were increased in the nuclear fraction of skeletal muscle. In additional experiments, we tested the role of glucocorticoids in sepsis-induced activation of C/EBP-beta and -delta by treating rats with the glucocorticoid receptor antagonist RU-38486. This treatment inhibited the sepsis-induced activation of C/EBP-beta and -delta , suggesting that glucocorticoids participate in the upregulation of C/EBP in skeletal muscle during sepsis. The present results suggest that C/EBP-beta and -delta are activated in skeletal muscle during sepsis and that this response is, at least in part, regulated by glucocorticoids.

transcription factors; cachexia; proteolysis; ubiquitin; proteasome; CCAAT/enhancer binding protein; deoxyribonucleic acid


This article has been cited by other articles:


Home page
 Physiol. Rev.Home page
M. J. Tisdale
Mechanisms of Cancer Cachexia
Physiol Rev, April 1, 2009; 89(2): 381 - 410.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
D. L. Allen, E. R. Bandstra, B. C. Harrison, S. Thorng, L. S. Stodieck, P. J. Kostenuik, S. Morony, D. L. Lacey, T. G. Hammond, L. L. Leinwand, et al.
Effects of spaceflight on murine skeletal muscle gene expression
J Appl Physiol, February 1, 2009; 106(2): 582 - 595.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
I. J. Smith, S. H. Lecker, and P.-O. Hasselgren
Calpain activity and muscle wasting in sepsis
Am J Physiol Endocrinol Metab, October 1, 2008; 295(4): E762 - E771.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
G. Fuster, S. Busquets, E. Ametller, M. Olivan, V. Almendro, C. C. Fontes de Oliveira, M. Figueras, F. J. Lopez-Soriano, and J. M. Argiles
Are Peroxisome Proliferator-Activated Receptors Involved in Skeletal Muscle Wasting during Experimental Cancer Cachexia? Role of {beta}2-Adrenergic Agonists
Cancer Res., July 1, 2007; 67(13): 6512 - 6519.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
H. Yang, W. Wei, M. Menconi, and P.-O. Hasselgren
Dexamethasone-induced protein degradation in cultured myotubes is p300/HAT dependent
Am J Physiol Regulatory Integrative Comp Physiol, January 1, 2007; 292(1): R337 - R334.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
W. Wei, H. Yang, M. Menconi, P. Cao, C. E. Chamberlain, and P.-O. Hasselgren
Treatment of cultured myotubes with the proteasome inhibitor beta-lactone increases the expression of the transcription factor C/EBPbeta
Am J Physiol Cell Physiol, January 1, 2007; 292(1): C216 - C226.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. GenomicsHome page
M. Salem, P. B. Kenney, C. E. Rexroad 3rd, and J. Yao
Microarray gene expression analysis in atrophying rainbow trout muscle: a unique nonmammalian muscle degradation model
Physiol Genomics, December 13, 2006; 28(1): 33 - 45.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
K. Ma, C. Mallidis, S. Bhasin, V. Mahabadi, J. Artaza, N. Gonzalez-Cadavid, J. Arias, and B. Salehian
Glucocorticoid-induced skeletal muscle atrophy is associated with upregulation of myostatin gene expression
Am J Physiol Endocrinol Metab, August 1, 2003; 285(2): E363 - E371.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online