AJP - Regu Information on EB 2010
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Regul Integr Comp Physiol 283: R698-R709, 2002. First published June 6, 2002; doi:10.1152/ajpregu.00039.2002
0363-6119/02 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
283/3/R698    most recent
00039.2002v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (51)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Frost, R. A.
Right arrow Articles by Lang, C. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Frost, R. A.
Right arrow Articles by Lang, C. H.
Vol. 283, Issue 3, R698-R709, September 2002

Lipopolysaccharide regulates proinflammatory cytokine expression in mouse myoblasts and skeletal muscle

Robert A. Frost, Gerald J. Nystrom, and Charles H. Lang

Department of Cellular and Molecular Physiology, The Pennsylvania State University, College of Medicine, Hershey, Pennsylvania 17033

The purpose of the present study was to examine the regulation of tumor necrosis factor (TNF)-alpha and interleukin (IL)-6 by lipopolysaccharide (LPS) in C2C12 myoblasts and mouse skeletal muscle. LPS produced dose- and time-dependent increases in TNF-alpha and IL-6 mRNA content in C2C12 myoblasts. The LPS-induced cytokine response could be mimicked by peptidoglycan from the cell wall of Staphylococcus aureus but not by zymosan A, a cell wall component from Saccharomyces cerevisiae. Ongoing protein synthesis was not necessary for the increase in the two cytokine mRNAs. The transcriptional inhibitor 5,6-dichloro-beta -D-ribofuranosyl-benzimidazole blocked LPS-stimulated IL-6 mRNA expression without changing its mRNA half-life. The anti-inflammatory glucocorticoid dexamethasone selectively blocked LPS-stimulated IL-6 mRNA accumulation but not TNF-alpha . In contrast, the proteasomal inhibitor MG-132 blocked TNF-alpha mRNA expression but not IL-6. Exposure of myoblasts to LPS was associated with a rapid decrease in the inhibitor of nuclear factor-kappa B (I kappa B, alpha , and epsilon ), and this response was also blocked by MG-132. Treatment of myocytes with IL-1 or TNF-alpha also increased IL-6 mRNA content, but the increase in IL-6 mRNA due to LPS could not be prevented by pretreatment with antagonists to either IL-1 or TNF. Under in vivo conditions, LPS increased the plasma concentration of TNF-alpha and IL-6 and stimulated the accumulation of their mRNAs in multiple tissues including skeletal muscle from wild-type mice. In contrast, the ability of LPS to stimulate the same cytokines was markedly decreased in mice that harbor a mutation in the Toll-like receptor 4. Our data suggest that LPS stimulates cytokine expression not only in classical immune tissues but also in skeletal muscle.

tumor necrosis factor; interleukin; dexamethasone; Toll-like receptor 4


This article has been cited by other articles:


Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
B. A. S. Borge, K.-H. Kalland, S. Olsen, A. Bletsa, E. Berggreen, and H. Wiig
Cytokines are produced locally by myocytes in rat skeletal muscle during endotoxemia
Am J Physiol Heart Circ Physiol, March 1, 2009; 296(3): H735 - H744.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
N. Dumont, P. Bouchard, and J. Frenette
Neutrophil-induced skeletal muscle damage: a calculated and controlled response following hindlimb unloading and reloading
Am J Physiol Regulatory Integrative Comp Physiol, December 1, 2008; 295(6): R1831 - R1838.
[Abstract] [Full Text] [PDF]

Home page
 Exp PhysiolHome page
K. A. Huey, G. Fiscus, A. F. Richwine, R. W. Johnson, and B. M. Meador
In vivo vitamin E administration attenuates interleukin-6 and interleukin-1{beta} responses to an acute inflammatory insult in mouse skeletal and cardiac muscle
Exp Physiol, December 1, 2008; 93(12): 1263 - 1272.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
B. K. Pedersen and M. A. Febbraio
Muscle as an Endocrine Organ: Focus on Muscle-Derived Interleukin-6
Physiol Rev, October 1, 2008; 88(4): 1379 - 1406.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
B. M. Meador, C. P. Krzyszton, R. W. Johnson, and K. A. Huey
Effects of IL-10 and age on IL-6, IL-1{beta}, and TNF-{alpha} responses in mouse skeletal and cardiac muscle to an acute inflammatory insult
J Appl Physiol, April 1, 2008; 104(4): 991 - 997.
[Abstract] [Full Text] [PDF]

Home page
 J ANIM SCIHome page
N. K. Gabler and M. E. Spurlock
Integrating the immune system with the regulation of growth and efficiency
J Anim Sci, April 1, 2008; 86(14_suppl): E64 - E74.
[Abstract] [Full Text] [PDF]

Home page
 J ANIM SCIHome page
R. A. Frost and C. H. Lang
Regulation of muscle growth by pathogen-associated molecules
J Anim Sci, April 1, 2008; 86(14_suppl): E84 - E93.
[Abstract] [Full Text] [PDF]

Home page
 Arch Otolaryngol Head Neck SurgHome page
T. Y. Cannon, D. Guttridge, J. Dahlman, J. R. George, V. Lai, C. Shores, P. Buzkova, and M. E. Couch
The Effect of Altered Toll-like Receptor 4 Signaling on Cancer Cachexia
Arch Otolaryngol Head Neck Surg, December 1, 2007; 133(12): 1263 - 1269.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
C. Weigert, M. Dufer, P. Simon, E. Debre, H. Runge, K. Brodbeck, H. U. Haring, and E. D. Schleicher
Upregulation of IL-6 mRNA by IL-6 in skeletal muscle cells: role of IL-6 mRNA stabilization and Ca2+-dependent mechanisms
Am J Physiol Cell Physiol, September 1, 2007; 293(3): C1139 - C1147.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
C. H. Lang, R. A. Frost, and T. C. Vary
Regulation of muscle protein synthesis during sepsis and inflammation
Am J Physiol Endocrinol Metab, August 1, 2007; 293(2): E453 - E459.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
M. Divangahi, A. Demoule, G. Danialou, L. Yahiaoui, W. Bao, Z. Xing, and B. J. Petrof
Impact of IL-10 on Diaphragmatic Cytokine Expression and Contractility during Pseudomonas Infection
Am. J. Respir. Cell Mol. Biol., April 1, 2007; 36(4): 504 - 512.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
J. H. Boyd, M. Divangahi, L. Yahiaoui, D. Gvozdic, S. Qureshi, and B. J. Petrof
Toll-Like Receptors Differentially Regulate CC and CXC Chemokines in Skeletal Muscle via NF-{kappa}B and Calcineurin
Infect. Immun., December 1, 2006; 74(12): 6829 - 6838.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
O. Prelovsek, T. Mars, M. Jevsek, M. Podbregar, and Z. Grubic
High dexamethasone concentration prevents stimulatory effects of TNF-{alpha} and LPS on IL-6 secretion from the precursors of human muscle regeneration
Am J Physiol Regulatory Integrative Comp Physiol, December 1, 2006; 291(6): R1651 - R1656.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
A. Demoule, M. Divangahi, L. Yahiaoui, G. Danialou, D. Gvozdic, K. Labbe, W. Bao, and B. J. Petrof
Endotoxin Triggers Nuclear Factor-{kappa}B-dependent Up-regulation of Multiple Proinflammatory Genes in the Diaphragm
Am. J. Respir. Crit. Care Med., September 15, 2006; 174(6): 646 - 653.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
N. Juretic, P. Garcia-Huidobro, J. A. Iturrieta, E. Jaimovich, and N. Riveros
Depolarization-induced slow Ca2+ transients stimulate transcription of IL-6 gene in skeletal muscle cells
Am J Physiol Cell Physiol, May 1, 2006; 290(5): C1428 - C1436.
[Abstract] [Full Text] [PDF]

Home page
 J ANIM SCIHome page
S. K. Jacobi, N. K. Gabler, K. M. Ajuwon, J. E. Davis, and M. E. Spurlock
Adipocytes, myofibers, and cytokine biology: New horizons in the regulation of growth and body composition
J Anim Sci, April 1, 2006; 84(13_suppl): E140 - E.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
R. A. Frost, G. J. Nystrom, and C. H. Lang
Multiple Toll-like receptor ligands induce an IL-6 transcriptional response in skeletal myocytes
Am J Physiol Regulatory Integrative Comp Physiol, March 1, 2006; 290(3): R773 - R784.
[Abstract] [Full Text] [PDF]

Home page
 J ANIM SCIHome page
B. J. Johnson, S. S. Dritz, K. A. Skjolaas-Wilson, T. E. Burkey, and J. E. Minton
Interactive responses in gut immunity, and systemic and local changes in the insulin-like growth factor system in nursery pigs in response to Salmonella enterica serovar Typhimurium
J Anim Sci, June 1, 2005; 83(13_suppl): E48 - 56.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
R. A. Frost, G. J. Nystrom, and C. H. Lang
Lipopolysaccharide stimulates nitric oxide synthase-2 expression in murine skeletal muscle and C2C12 myoblasts via Toll-like receptor-4 and c-Jun NH2-terminal kinase pathways
Am J Physiol Cell Physiol, December 1, 2004; 287(6): C1605 - C1615.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
M. H. S. Chan, A. L. Carey, M. J. Watt, and M. A. Febbraio
Cytokine gene expression in human skeletal muscle during concentric contraction: evidence that IL-8, like IL-6, is influenced by glycogen availability
Am J Physiol Regulatory Integrative Comp Physiol, August 1, 2004; 287(2): R322 - R327.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
R. A. Frost, G. J. Nystrom, and C. H. Lang
Epinephrine stimulates IL-6 expression in skeletal muscle and C2C12 myoblasts: role of c-Jun NH2-terminal kinase and histone deacetylase activity
Am J Physiol Endocrinol Metab, May 1, 2004; 286(5): E809 - E817.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
R. A. Frost, G. J. Nystrom, and C. H. Lang
Lipopolysaccharide and proinflammatory cytokines stimulate interleukin-6 expression in C2C12 myoblasts: role of the Jun NH2-terminal kinase
Am J Physiol Regulatory Integrative Comp Physiol, November 1, 2003; 285(5): R1153 - R1164.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
N. Qureshi, P.-Y. Perera, J. Shen, G. Zhang, A. Lenschat, G. Splitter, D. C. Morrison, and S. N. Vogel
The Proteasome as a Lipopolysaccharide-Binding Protein in Macrophages: Differential Effects of Proteasome Inhibition on Lipopolysaccharide-Induced Signaling Events
J. Immunol., August 1, 2003; 171(3): 1515 - 1525.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
G. Alexander, H. Carlsen, and R. Blomhoff
Strong In Vivo Activation of NF-{kappa}B in Mouse Lenses by Classic Stressors
Invest. Ophthalmol. Vis. Sci., June 1, 2003; 44(6): 2683 - 2688.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online