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1 Program in Neuroscience, The Florida State University, Tallahassee, Florida 32306; and 2 Department of Pharmacology, BK21 Project, Yonsei University College of Medicine, Seoul, South Korea
Food
deprivation (FD) increases hypothalamic neuropeptide Y (NPY) and
agouti-related protein (AGRP) mRNA levels and decreases proopiomelanocortin (POMC) mRNA levels; refeeding restores these levels. We determined the time course of changes in hypothalamic NPY,
AGRP, and POMC mRNA levels on refeeding after 24 h FD in C57BL
mice by in situ hybridization. After 24 h deprivation, mice were
refed with either chow or a palatable mash containing no calories or
were injected with murine leptin (100 µg) without food. Mice were
perfused 2 or 6 h after treatment. Food deprivation increased
hypothalamic NPY mRNA (108 ± 6%) and AGRP mRNA (78 ± 7%)
and decreased hypothalamic POMC mRNA (
15 ± 1%). Refeeding for
6 h, but not 2 h, was sufficient to reduce (but not restore) NPY mRNA, did not affect AGRP mRNA, and restored POMC mRNA levels to ad
libitum control levels. Intake of the noncaloric mash had no effect on
mRNA levels, and leptin administration after deprivation (at a dose
sufficient to reduce refeeding in FD mice) was not sufficient to affect
mRNA levels. These results suggest that gradual postabsorptive events
subsequent to refeeding are required for the restoration of peptide
mRNA to baseline levels after food deprivation in mice.
palatable; preabsorptive; postabsorptive; time course; arcuate nucleus; in situ hybridization; neuropeptide Y; agouti-related protein; proopiomelanocortin
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