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Am J Physiol Regul Integr Comp Physiol 284: R380-R388, 2003. First published October 17, 2002; doi:10.1152/ajpregu.00573.2002
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Vol. 284, Issue 2, R380-R388, February 2003

Active sulfate secretion by the intestine of winter flounder is through exchange for luminal chloride

Ryan M. Pelis and J. Larry Renfro

Department of Physiology and Neurobiology, University of Connecticut, Storrs, Connecticut 06269; and Mount Desert Island Biological Laboratory, Salisbury Cove, Maine 04672

SO<UP><SUB>4</SUB><SUP>2−</SUP></UP> transport by winter flounder intestine in Ussing chambers was characterized. With 50 mM SO<UP><SUB>4</SUB><SUP>2−</SUP></UP> (physiological level) bathing the lumen, net absorption (lumen to blood) dominated. Under short-circuited conditions, 1 mM SO<UP><SUB>4</SUB><SUP>2−</SUP></UP> on both sides, net active SO<UP><SUB>4</SUB><SUP>2−</SUP></UP> secretion occurred (8.55 ± 0.96 nmol · cm-2 · h-1). NaCN (10 mM), ouabain (10-4 M), and luminal DIDS (0.2 mM) inhibited net secretion. Removal of luminal Cl- and HCO<UP><SUB>3</SUB><SUP>−</SUP></UP> together (Cl--HCO<UP><SUB>3</SUB><SUP>−</SUP></UP>) or Cl- alone blocked net secretion, whereas removal of luminal HCO<UP><SUB>3</SUB><SUP>−</SUP></UP> alone increased net secretion. SO<UP><SUB>4</SUB><SUP>2−</SUP></UP> uptake into foregut brush-border membrane vesicles was stimulated by a trans-Cl- gradient (in > out) and unaffected by a trans-HCO<UP><SUB>3</SUB><SUP>−</SUP></UP> gradient (in > out). Short-circuiting with K+ (in = out) and valinomycin had no effect on Cl--stimulated SO<UP><SUB>4</SUB><SUP>2−</SUP></UP> uptake, suggesting electroneutral exchange. Satiety (i.e., full stomach) stimulated the unidirectional absorptive flux, eliminating net secretion. It was concluded that the intestine is a site of SO<UP><SUB>4</SUB><SUP>2−</SUP></UP> absorption in marine teleosts and that active SO<UP><SUB>4</SUB><SUP>2−</SUP></UP> secretion is in exchange for luminal Cl-.

Pleuronectes americanus; anion exchange; osmoregulation


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