AJP - Regu Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Regul Integr Comp Physiol 284: R1147-R1150, 2003; doi:10.1152/ajpregu.00448.2002
0363-6119/03 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (7)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Mrowka, R.
Right arrow Articles by Persson, P. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mrowka, R.
Right arrow Articles by Persson, P. B.
Vol. 284, Issue 4, R1147-R1150, April 2003

SPECIAL COMMUNICATION
An evolutionary approach for identifying potential transcription factor binding sites: the renin gene as an example

Ralf Mrowka, Karola Steinhage, Andreas Patzak, and Pontus B. Persson

Johannes-Müller-Institut für Physiologie, Charité, Humboldt-Universität zu Berlin, D-10117 Berlin, Germany

Evolutionary pressure has resulted in the conservation of certain nucleotide sequences. These conserved regions are potentially important for certain functions. Here we give an example of a comparison between noncoding sequences combined with other independent database information to shed light onto the regulation of the renin gene, a gene that has great importance for cardiovascular and renal homeostasis. To combine the information regarding conservation and weight matrices of transcription factor (TF) binding sites, an algorithm was developed (TFprofile). Notably, a local peak in the resulting binding profile coincides with a previously experimentally identified regulatory region for the renin gene. The existence of further peaks in the binding profile in the conserved 3.9-kb-long hRENc DNA block upstream of the renin gene suggests additional regions of potential importance for gene regulation. The algorithm TFprofile may be used to integrate information on cross-species evolutionary conservation and aspects of TF binding characteristics to provide putative regulatory DNA regions for experimental verification.

noncoding sequences; cross-species conservation


This article has been cited by other articles:


Home page
 Am. J. Physiol. Renal Physiol.Home page
L. M. Bivol, R. K. Berge, and B. M. Iversen
Differential effect of tetradecythioacetic acid on the renin-angiotensin system and blood pressure in SHR and 2-kidney, 1-clip hypertension
Am J Physiol Renal Physiol, September 1, 2007; 293(3): F839 - F845.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. GenomicsHome page
H. Castrop, M. Oppermann, Y. Weiss, Y. Huang, D. Mizel, H. Lu, S. Germain, F. Schweda, F. Theilig, S. Bachmann, et al.
Reporter gene recombination in juxtaglomerular granular and collecting duct cells by human renin promoter-Cre recombinase transgene
Physiol Genomics, April 13, 2006; 25(2): 277 - 285.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
P. B. Persson, A. Skalweit, R. Mrowka, and B.-J. Thiele
Control of renin synthesis
Am J Physiol Regulatory Integrative Comp Physiol, September 1, 2003; 285(3): R491 - R497.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online