AJP - Regu Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Regul Integr Comp Physiol 284: R1323-R1329, 2003. First published January 23, 2003; doi:10.1152/ajpregu.00756.2002
0363-6119/03 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
284/5/R1323    most recent
00756.2002v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (11)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ma, D.
Right arrow Articles by Bienenstock, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ma, D.
Right arrow Articles by Bienenstock, J.
Vol. 284, Issue 5, R1323-R1329, May 2003

Interleukin-10 and nerve growth factor have reciprocal upregulatory effects on intestinal epithelial cells

Donglai Ma1, Danielle Wolvers1,2, Andrzej M. Stanisz1, and John Bienenstock1

1 Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Ontario, Canada L8N 3Z5; and 2 Unilever Health Institute, Vlaardingen 3133 AT, The Netherlands

The intestinal mucosa is in a constant state of controlled inflammation, but the processes whereby this occurs are poorly understood. The aims of this study were to look at the role of IL-10 and nerve growth factor (NGF) in intestinal epithelial cell regulation. The human colon epithelial cell lines T84, HT-29, and CACO-2 were used. RT-PCR, flow cytometry analysis, and immunohistochemistry were applied to measure the cytokine changes in epithelial cells induced by recombinant cholera toxin and its B subunit, IL-10, and NGF. Cholera toxin B subunit caused selective dose-dependent increased mRNA for IL-10 in T84 cells and the protein in T84, HT-29, and CACO-2 cells. IL-10 dose dependently selectively increased NGF mRNA in T84 cells and intracellular protein synthesis in all three epithelial cell lines. The effect of NGF was reciprocal, selective, and dose dependent because it increased mRNA for IL-10 and IL-10 synthesis. Our results suggest that the epithelium may actively participate in downregulation through innate mechanisms involving IL-10 and NGF. The reciprocal interaction suggests for the first time that NGF may be involved in local downregulation by mucosal epithelium and thus may play a potent protective role in response to injury, by prevention of undue inflammation.

cholera toxin; cholera toxin B subunit


This article has been cited by other articles:


Home page
 GutHome page
T Kamiya, L Wang, P Forsythe, G Goettsche, Y Mao, Y Wang, G Tougas, and J Bienenstock
Inhibitory effects of Lactobacillus reuteri on visceral pain induced by colorectal distension in Sprague-Dawley rats
Gut, February 1, 2006; 55(2): 191 - 196.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
L. Bracci-Laudiero, L. Aloe, M. C. Caroleo, P. Buanne, N. Costa, G. Starace, and T. Lundeberg
Endogenous NGF regulates CGRP expression in human monocytes, and affects HLA-DR and CD86 expression and IL-10 production
Blood, November 15, 2005; 106(10): 3507 - 3514.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
D. Ma, P. Forsythe, and J. Bienenstock
Live Lactobacillus reuteri Is Essential for the Inhibitory Effect on Tumor Necrosis Factor Alpha-Induced Interleukin-8 Expression
Infect. Immun., September 1, 2004; 72(9): 5308 - 5314.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
M. Reinshagen and M. Steinkamp
NGF---not just a nerve growth factor in the gut
Am J Physiol Regulatory Integrative Comp Physiol, May 1, 2003; 284(5): R1322 - R1322.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online